Department of Laboratory Medicine, Clinical Center, Nationals Institutes of Health, Microbiology Service, Bldg 10/Rm 2C385, 10 Center Drive MSC 1508, Bethesda, MD, 20892-1508, USA.
Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2257-62. doi: 10.1007/s10096-012-1563-4. Epub 2012 Feb 28.
We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) for the rapid identification of anaerobic bacteria that had been isolated from clinical specimens and previously identified by 16s rRNA sequencing. The Bruker Microflex MALDI-TOF instrument with the Biotyper Software was used. We tested 152 isolates of anaerobic bacteria from 24 different genera and 75 different species. A total of 125 isolates (82%) had Biotyper software scores greater than 2.0 and the correct identification to genus and species was made by MALDI-TOF for 120 (79%) of isolates. Of the 12 isolates with a score between 1.8 and 2.0, 2 (17%) organisms were incorrectly identified by MALDI-TOF. Only 15 (10%) isolates had a score less than 1.8 and MALDI-TOF gave the wrong genus and species for four isolates, the correct genus for two isolates, and the correct genus and species for nine isolates. Therefore, we found the Bruker MALDI-TOF MicroFlex LT with an expanded database and the use of bacteria extracts rather than whole organisms correctly identified 130 of 152 (86%) isolates to genus and species when the cut-off for an acceptable identification was a spectrum score ≥1.8.
我们评估了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）在快速鉴定从临床标本中分离出的厌氧菌中的应用，这些厌氧菌先前已通过 16s rRNA 测序进行了鉴定。使用 Bruker Microflex MALDI-TOF 仪器和 Biotyper 软件。我们测试了来自 24 个不同属和 75 个不同种的 152 株厌氧菌。共有 125 株（82%）的 Biotyper 软件评分大于 2.0，MALDI-TOF 对 120 株（79%）的分离物进行了正确的属和种鉴定。在评分在 1.8 到 2.0 之间的 12 株中，有 2 株（17%）被 MALDI-TOF 错误鉴定。只有 15 株（10%）的分离物评分小于 1.8，MALDI-TOF 对 4 株分离物的属和种鉴定错误，对 2 株分离物的属鉴定正确，对 9 株分离物的属和种鉴定正确。因此，我们发现 Bruker MALDI-TOF MicroFlex LT 扩展了数据库，并使用细菌提取物而不是整个生物体，当可接受的鉴定谱评分≥1.8 时，正确鉴定了 152 株分离物中的 130 株（86%）至属和种。
