Dorostkar Kamran, Alavi-Shoushtari Sayed Mortaza, Mokarizadeh Aram
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2012 Fall;3(4):263-8.
The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 µg mL(-1) sodium selenite and the sperm motility and viability were evaluated at 0 (T0) (immediately after dilution), 60 (T1) and 120 (T2) min after diluting semen. In the second step, semen samples were diluted with tris-egg yolk-glycerol extender containing the same amounts of sodium selenite, cooled to 4 ˚C, equilibrated and semen parameters (motility, viability, membrane integrity and DNA damage) were estimated. Then, the semen was packed in 0.5 mL French straws and frozen in liquid nitrogen. Later, the semen was thawed and analyzed for the same parameters, as well as total antioxidant capacity. Results showed that addition of 1 and 2 µgmL(-1) selenium to the semen extender significantly increased the sperm motility of fresh and equilibrated semen compared to the control without affecting other parameters. However, in frozen-thawed semen, extenders containing 1 and 2 µg mL(-1) selenium significantly improved sperm motility, viability, membrane integrity and semen total antioxidant capacity and also resulted in lower DNA damaged sperms. In this study selenium supplementation of semen extender of 4 and 8 µg mL(-1) had deleterious effects on sperm parameters as early as the samples were prepared for freezing.
本研究的目的是调查体外补充硒对水牛公牛新鲜和冷冻精子质量的影响。使用了5头健康的水牛公牛（每头公牛采集5次射精样本）。每次射精样本在37˚C下用含有0（对照）、0.5、1、2、4和8 μg mL⁻¹亚硒酸钠的基于Tris的稀释液进行稀释，并在稀释精液后的0（T0）（稀释后立即）、60（T1）和120（T2）分钟评估精子活力和存活率。在第二步中，精液样本用含有相同量亚硒酸钠的Tris - 蛋黄 - 甘油稀释液进行稀释，冷却至4˚C，平衡后评估精液参数（活力、存活率、膜完整性和DNA损伤）。然后，将精液装入0.5 mL法式细管中并在液氮中冷冻。之后，将精液解冻并分析相同的参数以及总抗氧化能力。结果表明，与对照相比，在精液稀释液中添加1和2 μgmL⁻¹的硒显著提高了新鲜和平衡精液的精子活力，且不影响其他参数。然而，在冻融精液中，含有1和2 μg mL⁻¹硒的稀释液显著提高了精子活力、存活率、膜完整性和精液总抗氧化能力，并且还减少了DNA受损的精子。在本研究中，早在精液样本准备冷冻时，在精液稀释液中添加4和8 μg mL⁻¹的硒就对精子参数产生了有害影响。
