Horky Pavel, Urbankova Lenka, Bano Iqra, Kopec Tomas, Nevrkla Pavel, Pribilova Magdalena, Baholet Daria, Chilala Pompido, Slama Petr, Skalickova Sylvie
Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic.
Department of Physiology and Biochemistry, Faculty of Bio-Sciences, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, Nawabshah 67210, Pakistan.
Animals (Basel). 2023 Jul 30;13(15):2460. doi: 10.3390/ani13152460.
Selenium is an essential compound which can influence the fertility of boars by a greater margin. In past decades, research was mainly focused on a bioavailability of various selenium forms and the effect on semen quality. Recently, nanotechnology has expanded the possibilities of selenium supplementation research. Twenty-one Duroc boars (three groups with seven boars each) were included in this experiment with the first group being a control group with no selenium supplementation, and the second group being supplemented with 0.3 mg Se/kg of selenium in inorganic form of NaSeO. The third group was supplemented with selenium nanoparticles (100 nm) at the same dose as that of the second group. The experiment lasted for 126 days (three spermatogenesis cycles of boars) and the antioxidant parameters of boar semen were analysed at 42, 84 and 126 days, respectively. The antioxidant parameters (DPPH, FRAP, DMPD, GSH, GSSG) were not influenced by both SeNO and selenium nanoparticle supplementation during this experiment. At the end of the monitored period, significantly higher ( < 0.004) antioxidant readings were observed by using the ABTS method but not the DPPH, DMPD and FRAP methods on the supplemented groups compared to the control. Moreover, selenium-nanoparticle-supplemented groups showed elevated glutathione peroxidase activity in the seminal fluid ( < 0.008). However, the selenium nanoparticle supplementation has not shown an improving effect on sperm quality. This could be considered as a safe alternative to inorganic selenium as well as having a potential to enhance the antioxidant properties of the semen of boars.
硒是一种必需的化合物，它对公猪的生育能力有较大影响。在过去几十年中，研究主要集中在各种硒形态的生物利用度及其对精液质量的影响。最近，纳米技术拓展了硒补充研究的可能性。本实验纳入了21头杜洛克公猪（分为三组，每组7头），第一组为不补充硒的对照组，第二组以无机形式的亚硒酸钠（NaSeO）补充0.3毫克硒/千克。第三组以与第二组相同的剂量补充硒纳米颗粒（100纳米）。实验持续了126天（公猪的三个精子发生周期），分别在第42、84和126天对公猪精液的抗氧化参数进行了分析。在本实验期间，抗氧化参数（DPPH、FRAP、DMPD、GSH、GSSG）不受亚硒酸钠和硒纳米颗粒补充的影响。在监测期结束时，与对照组相比，使用ABTS法在补充组中观察到显著更高（<0.004）的抗氧化读数，但DPPH、DMPD和FRAP法未观察到。此外，补充硒纳米颗粒的组精液中的谷胱甘肽过氧化物酶活性升高（<0.008）。然而，补充硒纳米颗粒并未对精子质量显示出改善作用。这可被视为无机硒的一种安全替代物，并且有增强公猪精液抗氧化性能的潜力。
