Mishra Uma Kanta
Department of Anatomy, Histology and Embryology, Faculty of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, India.
Vet Res Forum. 2013 Summer;4(3):137-43.
Age related cytochemical changes of thymic endocrine cells were studied in 78 day old chicks at five day interval to age of day 60 employing a panel of cytochemical stains. Methenamine silver revealed cell morphology including cell processes distinctly while diamine silver revealed a stronger argentaffinity in these cells. The cells had greater affinity for diamine silver compared to methenamine silver (Argentaffin), followed by formaldehyde induced autofluorescence, argyrophilia, lead hematoxylin and HCl-toluidine. The chromaffin reaction was the weakest. Cytochemically, three different endocrine cell populations i.e. argentaffin cells, argyrophilic cells and amine precursor uptake and decarboxylation series (APUD)/chromaffin cells, formed the resident population of thymic endocrine cells. Occurrence of numerous serotonin storing cells, moderately frequent APUD cells, and fewer chromaffin as well as mast cells suggests for a conspicuous reservoir of amine storing cells in thymus. Morphologically argentaffin cells were of four types i.e. the peripherally granulated spherical cells (Type-I), densely granulated oval cells (Type-II), pyramidal argentaffin cells (Type-III) and diffusely granulated elongated cells (Type-IV). The type-II argentaffin cells were most frequent in the medulla followed by the type-I cells and the type-III cells. The type-IV cells were least in frequency. The age related changes in frequency of these cells are also discussed.
采用一组细胞化学染色方法，以5天为间隔，对78日龄雏鸡至60日龄的胸腺内分泌细胞的年龄相关细胞化学变化进行了研究。六胺银能清晰地显示细胞形态，包括细胞突起，而二胺银则显示这些细胞具有更强的嗜银性。与六胺银（嗜银性）相比，这些细胞对二胺银的亲和力更强，其次是甲醛诱导的自发荧光、嗜银性、铅苏木精和盐酸甲苯胺。嗜铬反应最弱。细胞化学上，三种不同的内分泌细胞群，即嗜银细胞、嗜银性细胞和胺前体摄取及脱羧系列（APUD）/嗜铬细胞，构成了胸腺内分泌细胞的常驻群体。大量储存5-羟色胺的细胞、中等频率的APUD细胞以及较少的嗜铬细胞和肥大细胞的存在表明胸腺中存在明显的胺储存细胞库。形态学上，嗜银细胞有四种类型，即外周颗粒状球形细胞（I型）、密集颗粒状椭圆形细胞（II型）、金字塔形嗜银细胞（III型）和弥漫性颗粒状细长细胞（IV型）。II型嗜银细胞在髓质中最常见，其次是I型细胞和III型细胞。IV型细胞频率最低。还讨论了这些细胞频率的年龄相关变化。
