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开发和鉴定巴氏豆丁海马（Halobatrachus didactylus）的新型微卫星标记。

Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus.

机构信息

MARE-Marine and Environmental Sciences Centre, ISPA-Instituto Universitário , Rua Jardim do Tabaco, Lisbon , Portugal.

Departamento de Biologia Animal and Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa , Campo Grande, Lisbon , Portugal.

出版信息

PeerJ. 2015 Jan 20;3:e731. doi: 10.7717/peerj.731. eCollection 2015.

DOI:10.7717/peerj.731
PMID:25653909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4304857/
Abstract

The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy-Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.

摘要

拉氏蟾鱼 Halobatrachus didactylus 是一种东大西洋多配偶制物种，具有雄性亲代照顾行为。本文描述了通过微卫星富集文库的 454 GS-FLX Titanium 焦磷酸测序获得的 5 个新的微卫星位点。每个多态性位点的等位基因数在 2 到 4 之间变化，观察到的杂合度范围从 0.082 到 0.600。没有发现偏离哈迪-温伯格平衡的显著偏差，也没有连锁不平衡的证据。这些标记将对该物种的亲子关系研究和种群遗传学具有重要价值。