Huang Liangzong, Ma Jun, Sun Yankuo, Lv Yanli, Lin Wen, Liu Mingjie, Tu Changsong, Zhou Pei, Gu Wanjun, Su Shuo, Zhang Guihong
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Arch Virol. 2015 Apr;160(4):979-85. doi: 10.1007/s00705-015-2351-0. Epub 2015 Feb 6.
Previous studies have demonstrated the key regulatory roles played by microRNAs (miRNAs) in influenza virus-host interactions. To gain more insight into the contribution of miRNAs to the host immune response, spleen tissues from mice infected with A/Swine/GD/2/12 (H1N1) virus were harvested 5 days postinfection, and miRNA deep sequencing was performed. The results showed that 50 miRNAs were modulated. Interestingly, pathway analysis of miRNAs and targets showed that upregulated miR-124-3p interacts with innate immune-related pathways such as the Toll-like receptor pathway, RIG-I-like receptor signaling pathway, NOD-like receptor signaling pathway and JAK-STAT signaling pathway, and this might play a major role in the anti-inflammatory response. Further understanding of the roles played by these miRNAs in influenza virus infection will provide new insights into host-pathogen interactions.
先前的研究已经证明了微小RNA（miRNA）在流感病毒与宿主相互作用中所起的关键调节作用。为了更深入地了解miRNA对宿主免疫反应的贡献，在感染A/猪/广东/2/12（H1N1）病毒5天后，从小鼠身上采集脾脏组织，并进行miRNA深度测序。结果显示有50种miRNA受到调控。有趣的是，对miRNA及其靶标的通路分析表明，上调的miR-124-3p与先天免疫相关通路相互作用，如Toll样受体通路、RIG-I样受体信号通路、NOD样受体信号通路和JAK-STAT信号通路，这可能在抗炎反应中起主要作用。进一步了解这些miRNA在流感病毒感染中所起的作用，将为宿主-病原体相互作用提供新的见解。
