Swanson R A, Sagar S M, Sharp F R
Neurology Service, V.A. Medical Canter, San Francisco, CA 94143.
Neurol Res. 1989 Mar;11(1):24-8. doi: 10.1080/01616412.1989.11739856.
Microwave fixation in situ was used to assess regional glycogen and glucose stores in normal rat brain. Glycogen levels were highest in the cerebellum and pons/medulla (38.0 and 35.6 nmol/mg protein), and lowest in the striatum and cerebral cortex (17.4 and 23.6 nmol/mg protein respectively). Glucose concentrations paralleled glycogen, ranging from 5.9 to 10.8 nmol/mg protein. Glycogen, glucose, and lactate were measured during complete global ischaemia (decapitation) to assess regional differences in ischaemic metabolism. Those regions which in normal brain contain higher glycogen and glucose stores were found to maintain significantly higher levels of glycogen and glucose for at least 2 minutes of ischaemia. Lactate accumulated to highest levels after 30 minutes of ischaemia in those regions with highest glucose and glycogen stores. Lactate levels did not, however, rise above 90 nmol/mg protein in any brain region, a level well below that considered potentially neurotoxic. The data indicate considerable regional differences in normal and ischaemic glycogen metabolism that might contribute to known regional differences in vulnerability to global ischaemia.
采用原位微波固定法评估正常大鼠脑内局部糖原和葡萄糖储备。糖原水平在小脑和脑桥/延髓最高（分别为38.0和35.6 nmol/mg蛋白质），在纹状体和大脑皮层最低（分别为17.4和23.6 nmol/mg蛋白质）。葡萄糖浓度与糖原平行，范围为5.9至10.8 nmol/mg蛋白质。在完全性全脑缺血（断头）期间测量糖原、葡萄糖和乳酸，以评估缺血代谢的局部差异。发现在正常脑中含有较高糖原和葡萄糖储备的那些区域，在缺血至少2分钟期间能维持显著较高水平的糖原和葡萄糖。在葡萄糖和糖原储备最高的那些区域，缺血30分钟后乳酸积累至最高水平。然而，在任何脑区乳酸水平均未升至90 nmol/mg蛋白质以上，该水平远低于被认为可能具有神经毒性的水平。数据表明正常和缺血糖原代谢存在相当大的局部差异，这可能导致已知的全脑缺血易损性的局部差异。
