通过单分子荧光共振能量转移直接观察微管的生长和收缩

Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer.

作者信息

Mondal Prasenjit, Chattoraj Shyamtanu, Chowdhury Rajdeep, Bhunia Debmalya, Ghosh Surajit, Bhattacharyya Kankan

机构信息

Chemistry Division, CSIR-Indian Institute of Chemical Biology, Jadavpur, Kolkata-700032, India.

出版信息

Phys Chem Chem Phys. 2015 Mar 14;17(10):6687-90. doi: 10.1039/c4cp06031h.

DOI:10.1039/c4cp06031h

PMID:25655478

Abstract

Single molecule Förster resonance energy transfer (FRET) has been applied, for the first time, to monitor the growth (polymerization) and the shrinkage (depolymerization) of the dynamic microtubules, employing EGFP (attached to Mal3) as a donor and alexa-568 bound to tubulin as an acceptor.

摘要

单分子荧光共振能量转移（FRET）首次被应用于监测动态微管的生长（聚合）和收缩（解聚），使用附着于Mal3的增强型绿色荧光蛋白（EGFP）作为供体，与微管蛋白结合的alexa - 568作为受体。