Tokumoto S, Hamamoto Y, Fujimoto K, Yamaguchi E, Okamura E, Honjo S, Ikeda H, Wada Y, Hamasaki A, Koshiyama H
Centre for Diabetes and Endocrinology, Tazuke Kofukai Foundation, Medical Research Institute, Kitano Hospital, Osaka, Japan.
Diabet Med. 2015 May;32(5):653-6. doi: 10.1111/dme.12696. Epub 2015 Feb 5.
To investigate the relationship between plasma betatrophin concentrations and insulin secretion capacity in people with Type 2 diabetes.
Glucagon stimulation tests (1 mg) were performed in 70 people with Type 2 diabetes after an overnight fast. Plasma betatrophin concentrations were measured using an enzyme-linked immunosorbent assay. Insulin secretion capacity was evaluated by measuring increments of C-peptide concentration in response to glucagon stimulation, and creatinine clearance was determined by comparing creatinine concentrations in serum and 24-h urine samples.
Plasma betatrophin concentrations were positively correlated with duration of Type 2 diabetes (r = 0.34, P = 0.003), and negatively correlated with increments of C-peptide concentration (r = 0.37, P = 0.001) and creatinine clearance (r = 0.37, P = 0.001). The correlation with increments of C-peptide concentration remained significant after adjustment for age and duration of Type 2 diabetes (r = 0.25, P = 0.037). Multivariate analysis identified age and increments of C-peptide concentration as independent factors associated with plasma betatrophin levels.
Plasma betatrophin levels inversely correlate with insulin secretion capacity, suggesting that betatrophin levels are regulated by insulin secretion capacity in humans.
研究2型糖尿病患者血浆β-促细胞生成素浓度与胰岛素分泌能力之间的关系。
70例2型糖尿病患者过夜禁食后进行胰高血糖素刺激试验（1毫克）。采用酶联免疫吸附测定法测量血浆β-促细胞生成素浓度。通过测量胰高血糖素刺激后C肽浓度的增加来评估胰岛素分泌能力，并通过比较血清和24小时尿液样本中的肌酐浓度来测定肌酐清除率。
血浆β-促细胞生成素浓度与2型糖尿病病程呈正相关（r = 0.34，P = 0.003），与C肽浓度增加呈负相关（r = 0.37，P = 0.001），与肌酐清除率呈负相关（r = 0.37，P = 0.001）。在调整年龄和2型糖尿病病程后，与C肽浓度增加的相关性仍然显著（r = 0.25，P = 0.037）。多变量分析确定年龄和C肽浓度增加是与血浆β-促细胞生成素水平相关的独立因素。
血浆β-促细胞生成素水平与胰岛素分泌能力呈负相关，提示在人类中β-促细胞生成素水平受胰岛素分泌能力调节。
