Peng Li, Chen Chen, Kai-yi Han, Feng-xia Zhang, Yan-li Zhu, Zong-shuai Ling, Xing-xiao Zhang, Shi-jin Jiang, Zhi-jing Xie
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian 271018, Shandong, China; College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, Shandong, China.
College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, Shandong, China.
Vet Microbiol. 2015 Mar 23;176(1-2):88-96. doi: 10.1016/j.vetmic.2015.01.009. Epub 2015 Jan 15.
In mid-August 2013, two H9N2 influenza viruses, named A/mink/Shandong/F6/2013 (Mk/SD/F6/13) and A/mink/Shandong/F10/2013 (Mk/SD/F10/13), were isolated from lung samples of 2 of 45 farmed mink exhibiting respiratory signs in mideastern Shandong province, China. The seroprevalence of antibodies to H9N2 in mink was 20% (53/265). Based on sequence analysis, the eight nucleotide sequences showed 99.7-100% identity between Mk/SD/F6/13 and Mk/SD/F10/13. The HA, NP and NS genes of Mk/SD/F6/13 and Mk/SD/F10/13 were close to A/chicken/Zhejiang/329/2011 (H9N2), the NA and PB1 genes to A/duck/Hunan/S4111/2011 (H9N2), the PA and M genes to A/chicken/Shanghai/C1/2012 (H9N2). However, the PB2 genes had a close relationship with A/Turkey/California/189/66 (H9N2). Based on Sialic acid (SA) receptor detection, a range tissues of the mink demonstrated staining for MAA and/or SNA, and mink could serve as an intermediate host for influenza viruses with pandemic potential for the other animals. Experimental infection of mink demonstrated that mink could be infected by H9N2 influenza viruses and presented mild clinical signs, virus shedding and seroconversion, but no animals died of the disease. It implied that mammalian host-adapted avian H9N2 strains infected mink.
2013年8月中旬，从中国山东省中东部45只出现呼吸道症状的养殖水貂中的2只的肺样本中分离出两种H9N2流感病毒，分别命名为A/水貂/山东/F6/2013（Mk/SD/F6/13）和A/水貂/山东/F10/2013（Mk/SD/F10/13）。水貂中H9N2抗体的血清阳性率为20%（53/265）。基于序列分析，8个核苷酸序列显示Mk/SD/F6/13和Mk/SD/F10/13之间的同一性为99.7 - 100%。Mk/SD/F6/13和Mk/SD/F10/13的HA、NP和NS基因与A/鸡/浙江/329/2011（H9N2）接近，NA和PB1基因与A/鸭/湖南/S4111/2011（H9N2）接近，PA和M基因与A/鸡/上海/C1/2012（H9N2）接近。然而，PB2基因与A/土耳其/加利福尼亚/189/66（H9N2）关系密切。基于唾液酸（SA）受体检测，水貂的一系列组织显示出对MAA和/或SNA的染色，并且水貂可以作为对其他动物具有大流行潜力的流感病毒的中间宿主。水貂的实验性感染表明，水貂可被H9N2流感病毒感染，并表现出轻度临床症状、病毒脱落和血清转化，但没有动物死于该疾病。这表明适应哺乳动物宿主的禽H9N2毒株感染了水貂。
