Choo S H, Sung H H, Chae M R, Kang S J, Han D H, Park J K, So I, Lee S W
Department of Urology, Samsung Medical Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, South Korea; Department of Urology, Ajou University School of Medicine, Suwon, South Korea.
Andrology. 2015 Mar;3(2):309-14. doi: 10.1111/andr.12004. Epub 2015 Feb 5.
Although there are several methods for assessing erectile function in rats, the standard methods for telemetric monitoring have not been established. Theoretically assessment of spontaneous erection (SE) seems to be a physiologic method but it needs long measuring time and additional efforts. Apomorphine-induced erection (AIE) is one available and simple method; however, the correlation with SE has not been assessed. We compared erection profiles of AIE and SE in normal and two disease rat models using telemetric assessment of intracavernosal pressure (ICP). Seven-week-old male Sprague-Dawley rats were assigned to normal control, diabetes mellitus (DM) and hypercholesterolemia (HC) group. After 19 weeks a telemetric pressure sensor (C40; Data Sciences) was surgically implanted in the corpus cavernosum. One week later, ICP was recorded in freely moving rats after intraperitoneal apomorphine (100 μg/kg) injection (AIE) or during SE. Sexual events were visually identified and recorded. Only the pressure increases that occurred during sexual behavior were analyzed. We compared the erectile profiles such as duration, maximal ICP and the area under the curve (AUC, area under time × ICP curves). Two-way anova revealed no significant effect of the measuring methods on the mean AUC (F1,43 = 2.756, p-value = 0.104), but a significant effect of different disease models on mean AUC (two-way anova: F2,43 = 12.929, p-value < 0.001) was observed. The mean AUC of normal control rats was significantly higher than that of DM and HC rats (Bonferroni post hoc test: p < 0.001 and p = 0.001, respectively). ICP measurements using a telemetric device showed no significant difference in AUC between AIE and SE. AIE is easy and requires less time than SE measurements. Therefore, AIE could be a useful method to evaluate ICP in rats.
虽然有几种评估大鼠勃起功能的方法，但用于遥测监测的标准方法尚未确立。理论上，对自发勃起（SE）的评估似乎是一种生理学方法，但它需要较长的测量时间和额外的努力。阿扑吗啡诱导勃起（AIE）是一种可行且简单的方法；然而，它与SE的相关性尚未得到评估。我们使用海绵体内压（ICP）的遥测评估，比较了正常和两种疾病大鼠模型中AIE和SE的勃起情况。将7周龄雄性Sprague-Dawley大鼠分为正常对照组、糖尿病（DM）组和高胆固醇血症（HC）组。19周后，通过手术将遥测压力传感器（C40；Data Sciences）植入海绵体。一周后，在腹腔注射阿扑吗啡（100μg/kg）后（AIE）或在SE期间，记录自由活动大鼠的ICP。通过视觉识别并记录性行为事件。仅分析性行为期间发生的压力升高。我们比较了勃起情况，如持续时间、最大ICP和曲线下面积（AUC，时间×ICP曲线下面积）。双向方差分析显示测量方法对平均AUC无显著影响（F1,43 = 2.756，p值 = 0.104），但观察到不同疾病模型对平均AUC有显著影响（双向方差分析：F2,43 = 12.929，p值 < 0.001）。正常对照大鼠的平均AUC显著高于DM和HC大鼠（Bonferroni事后检验：分别为p < 0.001和p = 0.001）。使用遥测设备进行的ICP测量显示，AIE和SE之间的AUC无显著差异。AIE操作简便，比SE测量所需时间更少。因此，AIE可能是评估大鼠ICP的一种有用方法。
