Qiao X-H, Zhang J-J, Gao F, Li F, Liu Y, Xing L-X, Du L-F, Xing J-F
Department of Medical Ultrasound, Shanghai General Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.
Int J Impot Res. 2017 Jan;29(1):12-16. doi: 10.1038/ijir.2016.37. Epub 2016 Sep 22.
The aim of this study was to investigate the feasibility of two-dimensional-ShearWave™ Elastography (2D-SWE) on evaluating the change of tissue structure of penis. Twenty healthy male Sprague Dawley rats were divided into penis-developed group (PDG, 52 weeks) and penis-underdeveloped group (PUDG, 5 weeks). The ultrafast ultrasound device-Aixplorer® (SuperSonic Imagine) was used for 2D-SWE imaging of the penis, the measurement index was shear wave stiffness (SWS, kPa). All rat penises were cut off immediately after ultrasonic examination. After paraffin embedding, slicing and hematoxylin-eosin staining, the tissue structure of the penis was observed under light microscope. SWS of all rat penises were measured successfully. The results showed that SWS of PDG was significantly lower than PUDG (P=0.008). At the same time, the pathological results found that there were significant differences in the tissue structures (sinusoids, smooth muscle cells and fibrocytes) of the penises between the two groups. These results suggest that there are significant differences in SWS between different tissue structures of penis. 2D-SWE is expected to be used on the etiological diagnosis of erectile dysfunction by serving as a new noninvasive method of evaluating the change of tissue structure of penis.
本研究旨在探讨二维剪切波弹性成像（2D-SWE）评估阴茎组织结构变化的可行性。将20只健康雄性Sprague Dawley大鼠分为阴茎发育组（PDG，52周）和阴茎发育不全组（PUDG，5周）。使用超高速超声设备Aixplorer®（Supersonic Imagine）对阴茎进行2D-SWE成像，测量指标为剪切波硬度（SWS，kPa）。超声检查后立即切除所有大鼠的阴茎。经过石蜡包埋、切片和苏木精-伊红染色后，在光学显微镜下观察阴茎的组织结构。成功测量了所有大鼠阴茎的SWS。结果显示，PDG的SWS显著低于PUDG（P = 0.008）。同时，病理结果发现两组阴茎的组织结构（血窦、平滑肌细胞和成纤维细胞）存在显著差异。这些结果表明，阴茎不同组织结构的SWS存在显著差异。2D-SWE有望作为一种评估阴茎组织结构变化的新型无创方法，用于勃起功能障碍的病因诊断。
