Sandin Marianne, Chawade Aakash, Levander Fredrik
Department of Immunotechnology, Lund University, Lund, Sweden.
Proteomics Clin Appl. 2015 Apr;9(3-4):289-94. doi: 10.1002/prca.201400202. Epub 2015 Mar 21.
Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label-free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of label-free techniques by the proteomics community in the near future.
无标记液相色谱-质谱方法对于高通量定量蛋白质组学具有吸引力，因为样品处理简单直接，并且可以扩展到大量样品。因此，无标记方法有助于在涉及数十个临床样品的研究中发现生物标志物。然而，尽管无标记工作流程越来越受欢迎，但研究界在临床蛋白质组学研究中使用它仍存在犹豫。因此，我们在此讨论用于生物标志物发现的无标记液相色谱-串联质谱的优缺点，并阐述其成功应用的主要前提条件。此外，我们引用了无标记液相色谱-串联质谱成功用于鉴定新型生物标志物的研究，并预计在不久的将来蛋白质组学界对无标记技术的接受度会提高。
