用于血清和细胞提取物中前列腺特异性抗原的荧光共振能量转移免疫分析的生物共轭持久发光纳米颗粒，无需原位激发。
Bioconjugated persistent luminescence nanoparticles for Föster resonance energy transfer immunoassay of prostate specific antigen in serum and cell extracts without in situ excitation.
作者信息
Wu Bo-Yue, Yan Xiu-Ping
机构信息
College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 30071, China.
出版信息
Chem Commun (Camb). 2015 Mar 4;51(18):3903-6. doi: 10.1039/c5cc00286a.
A novel Föster resonance energy transfer (FRET) immunoassay based on persistent luminescence nanoparticles (PLNP) was established for prostate specific antigen (PSA) detection in serum and cell extracts without in situ excitation. The specific FRET behavior allows highly selective and sensitive ratiometric photoluminescent detection of PSA in biological samples.
建立了一种基于持久发光纳米颗粒（PLNP）的新型荧光共振能量转移（FRET）免疫分析方法，用于在无需原位激发的情况下检测血清和细胞提取物中的前列腺特异性抗原（PSA）。这种特定的FRET行为能够对生物样品中的PSA进行高选择性和高灵敏度的比率光致发光检测。
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