de Assis Jorgiana Silva, Lima Ramille Araujio, Marques Lima Juliana Paiva, Azevedo Rodrigues Lidiany Karle, Santiago Sérgio Lima
Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.
J Conserv Dent. 2015 Jan-Feb;18(1):51-5. doi: 10.4103/0972-0707.148896.
Epigallocatechin-3-gallate (EGCG) is a flavonoid extracted from green tea that demonstrated antimicrobial activity.
To evaluate the efficacy of EGCG 0.5%, 1%, and 2% concentrations as an antimicrobial solution in dentin caries-like lesions induced in a bacterial-based in vitro model.
Twenty-five human dentin specimens were submitted to a microbial-based caries model by immersion in brain heart infusion (BHI) broth inoculated with Streptococcus mutans UA159, for 5 days. After the demineralization period, the specimens were randomly divided into groups: Group I: 0.9% saline solution; Group II: 2% chlorhexidine digluconate; Group III: 0.5% EGCG; Group IV: 1% EGCG; and Group V: 2% EGCG. After the treatments, carious dentin samples were harvested from dentin specimens and analyzed by colony-forming unit (CFU) counts. Data were analyzed by ANOVA and Tukey's test.
Log reduction values (SD, CFU.mg(-1)) for Groups I-V were: 5.02 (0.16), 3.96 (0.43), 4.74 (0.26), 4.89 (0.56), and 4.91 (0.40), respectively. There was no statistical difference between the EGCG concentrations and saline solution (P > 0.05). Furthermore, there was no statistical difference between EGCG concentrations (P > 0.05). However, there was a statistically significant difference between the chlorhexidine digluconate group and the other groups (P < 0.05).
EGCG at the studied concentrations were not effective in eliminating S. mutans from dentin caries-like lesions.
表没食子儿茶素-3-没食子酸酯（EGCG）是从绿茶中提取的一种黄酮类化合物，具有抗菌活性。
在基于细菌的体外模型中，评估浓度为0.5%、1%和2%的EGCG作为抗菌溶液对牙本质龋样病变的疗效。
将25个人类牙本质标本浸入接种变形链球菌UA159的脑心浸液（BHI）肉汤中5天，建立基于微生物的龋病模型。脱矿期结束后，将标本随机分为几组：第一组：0.9%生理盐水溶液；第二组：2%葡萄糖酸氯己定；第三组：0.5%EGCG；第四组：1%EGCG；第五组：2%EGCG。处理后，从牙本质标本中采集龋坏牙本质样本，通过菌落形成单位（CFU）计数进行分析。数据采用方差分析和Tukey检验进行分析。
第一组至第五组的对数减少值（标准差，CFU.mg⁻¹）分别为：5.02（0.16）、3.96（0.43）、4.74（0.26）、4.89（0.56）和4.91（0.40）。EGCG各浓度组与生理盐水组之间无统计学差异（P>0.05）。此外，EGCG各浓度组之间也无统计学差异（P>0.05）。然而，葡萄糖酸氯己定组与其他组之间存在统计学显著差异（P<0.05）。
在所研究的浓度下EGCG对从牙本质龋样病变中清除变形链球菌无效。
