School of Dentistry, National Taiwan University, Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
J Formos Med Assoc. 2018 Apr;117(4):276-282. doi: 10.1016/j.jfma.2018.01.020. Epub 2018 Feb 12.
BACKGROUND/PURPOSE: Fluoride and epigallocatechin gallate (EGCG) have been proven to prevent dental caries. The purpose of this study was to evaluate the effects of fluoride and EGCG on soft-drink-induced dental erosion in vitro.
Forty enamel and dentin specimens were prepared from extracted human teeth. The specimens were divided into 4 groups and treated separately with distilled water (as control), 0.5 M sodium fluoride (NF), 400 μM EGCG (EG), and a solution containing 0.5 M NaF and 400 μM EGCG (FG). Cyclic erosive treatment was performed according to the experimental procedures. The specimens were analyzed using laser scanning confocal microscopy, scanning electron microscopy, and a microhardness tester. The data were analyzed using ANOVA and Bonferroni's post hoc test. The significance level was set at 5%.
The amount of substance loss was lower in the NF and EG groups than in the control group (p < 0.05). The erosion-caused substance loss was more pronounced in the dentin than in the enamel specimens. Surface microhardness loss was lower in the NF and EG groups than in the control group (p < 0.05). The diameter of the dentinal tubule was wider in the control group than in the NF and EG groups (p < 0.05). No combined effects were observed in the FG group.
Both fluoride and EGCG are effective in preventing soft-drink-induced erosion compared with the control group. Fluoride and EGCG may interfere with each other. The mechanisms of the anti-erosive effect need to be explored in the future.
背景/目的：氟化物和表没食子儿茶素没食子酸酯（EGCG）已被证明可预防龋齿。本研究旨在评估氟化物和 EGCG 对软饮料诱导的牙釉质和牙本质侵蚀的体外影响。
从人离体牙中制备 40 个牙釉质和牙本质标本。将标本分为 4 组，分别用蒸馏水（作为对照）、0.5M 氟化钠（NF）、400μM EGCG（EG）和含有 0.5M NaF 和 400μM EGCG（FG）的溶液处理。根据实验程序进行循环腐蚀性处理。使用激光扫描共聚焦显微镜、扫描电子显微镜和显微硬度计分析标本。使用方差分析和 Bonferroni 事后检验分析数据。显著性水平设定为 5%。
NF 和 EG 组的物质损失量低于对照组（p<0.05）。与牙釉质标本相比，牙本质标本的侵蚀性物质损失更为明显。NF 和 EG 组的表面显微硬度损失低于对照组（p<0.05）。与 NF 和 EG 组相比，对照组的牙本质小管直径更宽（p<0.05）。FG 组未观察到联合效应。
与对照组相比，氟化物和 EGCG 均可有效预防软饮料诱导的侵蚀。氟化物和 EGCG 可能相互干扰。未来需要探索其抗侵蚀作用的机制。
