Daugherty Ana M, Yu Qijing, Flinn Robert, Ofen Noa
Institute of Gerontology, Wayne State University, Detroit, MI 48202, USA.
Institute of Gerontology, Wayne State University, Detroit, MI 48202, USA; Department of Psychology, Wayne State University, Detroit, MI 48202, USA.
Int J Dev Neurosci. 2015 Apr;41:115-22. doi: 10.1016/j.ijdevneu.2015.02.001. Epub 2015 Feb 7.
There is a growing interest in characterizing functional specialization along the long axis of the hippocampus in humans. Variability in volumetry along the long axis of the hippocampus may be of functional relevance in human development, and in a number of clinical populations. However, there is a lack of consistent definitions for measurements of regional volumetry along the hippocampal long axis. Moreover, there is lack of consistent reliability standards of these measures. Here we describe a protocol for manual demarcation of hippocampal head, body, and tail. The definitions emphasize anatomical landmarks that agree with the extant literature and are visible in children and adults alike. Using this protocol we achieved high reliability of all volumetric measures. We further demonstrate that the protocol can be applied to T2-weighted images optimized for high-resolution scanning of the hippocampus, as well as a more standard T1-weighted image sequence. Third, the protocol is sensitive to detect individual differences in subregion volumes in normally developing children (N=81; ages 8-25 years). This protocol may be of use for researchers studying the hippocampus across the lifespan and in diverse clinical populations.
人们对描述人类海马体长轴上的功能特化越来越感兴趣。海马体长轴上体积测量的变异性可能在人类发育以及一些临床人群中具有功能相关性。然而，对于沿海马体长轴的区域体积测量缺乏一致的定义。此外，这些测量缺乏一致的可靠性标准。在此，我们描述了一种手动划分海马体头部、体部和尾部的方案。这些定义强调了解剖学标志，这些标志与现有文献一致，并且在儿童和成人中都可见。使用该方案，我们实现了所有体积测量的高可靠性。我们进一步证明，该方案可应用于为海马体高分辨率扫描优化的T2加权图像，以及更标准的T1加权图像序列。第三，该方案能够灵敏地检测正常发育儿童（N = 81；年龄8 - 25岁）亚区域体积的个体差异。该方案可能对研究不同年龄段以及不同临床人群中海马体的研究人员有用。
