预测食管小细胞癌患者术后预后的微小RNA谱

MicroRNA profiles to predict postoperative prognosis in patients with small cell carcinoma of the esophagus.

作者信息

Okumura Tomoyuki, Shimada Yutaka, Omura Tetsuya, Hirano Katsuhisa, Nagata Takuya, Tsukada Kazuhiro

机构信息

Department of Surgery and Science, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Department of Surgery and Science, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan Department of Nanobio Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.

出版信息

Anticancer Res. 2015 Feb;35(2):719-27.

PMID:25667451

Abstract

BACKGROUND/AIM: Small cell carcinoma of the esophagus (SCCE) is a rare but very aggressive disease with poor prognosis. The aim of the present study was to identify a molecular signature to predict postoperative outcomes in patients with SCCE.

MATERIALS AND METHODS

Expression of microRNA was detected in surgically-removed SCCE tumors using microarrays. A SCCE cell line (TYUC-1) was established to investigate the biological role of differentially expressed microRNAs.

RESULTS

Hierarchical clustering of microRNA expression revealed two discrete clusters that were identical to the cases with rapid tumor relapse (n=3; median survival, 5.1 months) and the cases with long-term survival (n=3; median observation, 144.7 months), respectively. Eight microRNAs (miR-4323, miR-625, miR-3619-3p, miR-4419b, miR-1249, miR-4648, miR-4664-3p and miR-1203) showed significant correlation with tumor relapse (p<0.01). Migration of TYUC-1 was significantly inhibited by down-regulation of miR-625.

CONCLUSION

The expression profiles of microRNAs in tumors may represent a novel predictor for postoperative outcomes in patients with SCCE.

摘要

背景/目的：食管小细胞癌（SCCE）是一种罕见但极具侵袭性的疾病，预后较差。本研究的目的是确定一种分子特征，以预测SCCE患者的术后结局。

材料与方法

使用微阵列检测手术切除的SCCE肿瘤中微小RNA的表达。建立了SCCE细胞系（TYUC-1）以研究差异表达的微小RNA的生物学作用。

结果

微小RNA表达的层次聚类揭示了两个不同的聚类，分别与肿瘤快速复发的病例（n = 3；中位生存期，5.1个月）和长期生存的病例（n = 3；中位观察期，144.7个月）相同。八个微小RNA（miR-4323、miR-625、miR-3619-3p、miR-4419b、miR-1249、miR-4648、miR-4664-3p和miR-1203）与肿瘤复发显著相关（p<0.01）。miR-625的下调显著抑制了TYUC-1的迁移。