†Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
‡College of Chemistry, Jilin University, Changchun 130012, China.
ACS Nano. 2015 Mar 24;9(3):3199-205. doi: 10.1021/acsnano.5b00276. Epub 2015 Feb 13.
A protease-activated ratiometric fluorescent probe based on fluorescence resonance energy transfer between a pH-sensitive fluorescent dye and biocompatible Fe3O4 nanocrystals was constructed. A peptide substrate of MMP-9 served as a linker between the particle quencher and the chromophore that was covalently attached to the antitumor antibody. The optical response of the probe to activated MMP-9 and gastric cell line SGC7901 tumor cells was investigated, followed by in vivo tumor imaging. Based on the ratiometric pH response to the tumor microenvironment, the resulting probe was successfully used to image the pH of subcutaneous tumor xenografts.
构建了一种基于 pH 敏感荧光染料与生物相容性 Fe3O4 纳米晶体之间荧光共振能量转移的蛋白酶激活比率荧光探针。MMP-9 的肽底物作为颗粒猝灭剂与共价连接到抗肿瘤抗体的生色团之间的连接物。研究了探针对激活的 MMP-9 和胃细胞系 SGC7901 肿瘤细胞的光学响应，随后进行了体内肿瘤成像。基于对肿瘤微环境的比率 pH 响应，成功地使用所得探针对皮下肿瘤异种移植物的 pH 进行成像。
