Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
ACS Nano. 2010 Dec 28;4(12):7467-74. doi: 10.1021/nn102644u. Epub 2010 Nov 17.
A dual-emission nanoprobe that can sense changes in the environmental pH is designed based on the concept of pH-responsive Förster resonance energy transfer induced by the conformational transition of an associating polyelectrolyte, N-palmitoyl chitosan, bearing a donor (Cy3) or an acceptor (Cy5) moiety. We demonstrate that the developed pH-responsive nanoprobe can be used to ratiometrically image and thus discriminate the pH changes in the biological environment at different length scales.
设计了一种基于具有供体（Cy3）或受体（Cy5）部分的缔合聚电解质 N-棕榈酰壳聚糖构象转变诱导的环境 pH 响应Förster 共振能量转移概念的双发射纳米探针，以感应环境 pH 的变化。我们证明，所开发的 pH 响应纳米探针可用于比率成像，从而在不同长度尺度上区分生物环境中的 pH 变化。
