Rodríguez F, Ball H J, Suárez-Bonnet A, Ramírez A S, Fernández A
Units of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.
Department of Agriculture for Northern Ireland, Veterinary Sciences Division, Stoney Road, Stormont, Belfast, Northern Ireland, UK.
J Comp Pathol. 2015 Feb-Apr;152(2-3):106-9. doi: 10.1016/j.jcpa.2014.12.001. Epub 2015 Feb 7.
The pathogenesis and persistence of Mycoplasma bovis (Mb) infection of the respiratory tract is incompletely understood. Cyclooxygenase (COX)-2 is overexpressed during inflammatory responses by different cell types in the lung. This study evaluated COX-2 expression immunohistochemically in the inflammatory lesions of calves with naturally occurring and experimentally induced Mb pneumonia. Experimentally infected lungs showed catarrhal bronchointerstitial pneumonia and varying degrees of peribronchiolar mononuclear cell cuffing. Lesions in calves with spontaneously arising disease included exudative bronchopneumonia and extensive foci of coagulative necrosis surrounded by inflammatory cells. Mb antigen was located in epithelial and inflammatory cells in the airway lumina and surrounding areas of necrosis. COX-2 protein was detected in the lung of all infected calves and was localized to goblet cells, bronchial, bronchiolar and alveolar epithelial cells and macrophages. COX-2 protein was overexpressed during Mb infection and was always associated with areas of pneumonia and with the presence of Mb antigen.
牛支原体（Mb）呼吸道感染的发病机制和持续存在尚未完全明确。环氧化酶（COX）-2在肺部不同细胞类型的炎症反应过程中过度表达。本研究采用免疫组织化学方法评估了自然发生和实验性诱导的Mb肺炎犊牛炎症病变中COX-2的表达情况。实验感染的肺脏表现为卡他性支气管间质性肺炎和不同程度的支气管周围单核细胞套袖状浸润。自然发病犊牛的病变包括渗出性支气管肺炎和被炎性细胞包围的广泛凝固性坏死灶。Mb抗原位于气道管腔和坏死周围区域的上皮细胞和炎性细胞中。在所有感染犊牛的肺脏中均检测到COX-2蛋白，其定位于杯状细胞、支气管、细支气管和肺泡上皮细胞以及巨噬细胞。在Mb感染期间COX-2蛋白过度表达，并且总是与肺炎区域以及Mb抗原的存在相关。
