Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo, Japan.
Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo, Japan.
J Comp Pathol. 2020 Apr;176:14-18. doi: 10.1016/j.jcpa.2020.01.005. Epub 2020 Feb 27.
Lung tissues from calves infected experimentally with Mycoplasma bovis were examined by immunohistochemistry and electron microscopy. All inoculated calves had dark red areas of consolidation affecting both left and right lungs, which were characterized microscopically by subacute purulent bronchiolitis with hyperplasia of the surrounding lymphoid tissue. Immunohistochemically, M. bovis antigen was detected on the surface and inside the cytoplasm of bronchiolar epithelial cells in the pneumonic foci. The antigen was also found in the cytoplasm of phagocytes at the margin of bronchiolar exudates. Electron microscopically, numerous organisms were demonstrated in the immunohistochemically-positive sites. These findings suggest that M. bovis organisms adhere to the bronchiolar epithelium and at least some of them invade the epithelium.
用免疫组织化学和电子显微镜检查了实验感染牛支原体的小牛的肺组织。所有接种的小牛的左右肺都有暗红色实变区，显微镜下表现为亚急性化脓性细支气管炎，周围淋巴组织增生。免疫组织化学检查，在肺炎灶的细支气管上皮细胞表面和细胞质内检测到牛支原体抗原。抗原也存在于细支气管渗出物边缘的吞噬细胞细胞质中。电镜下，在免疫组织化学阳性部位显示出许多微生物。这些发现表明，牛支原体微生物附着在细支气管上皮上，其中至少有一些微生物侵入上皮。
