Rodríguez F, Bryson D G, Ball H J, Forster F
Department of Animal Pathology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Spain.
J Comp Pathol. 1996 Aug;115(2):151-62. doi: 10.1016/s0021-9975(96)80037-5.
Lung tissues from calves naturally and experimentally infected with Mycoplasma bovis were subjected to histopathological and immunohistochemical examination. The latter was carried out with a monoclonal antibody raised against M. bovis, and an avidin-biotin-peroxidase complex (ABC) detection substrate system. Pulmonary lesions in naturally infected calves included exudative bronchopneumonia and extensive foci of coagulative necrosis surrounded by inflammatory cells. Experimentally infected lungs showed suppurative bronchiolitis and varying degrees of peribronchiolar mononuclear cell cuffing. M. bovis antigen in field cases was mainly detected at the periphery of the areas of coagulative necrosis, in necrotic exudates, and in close association with infiltrating macrophages and neutrophils. In lung tissue from calves with induced M. bovis pneumonia, antigen was located in epithelial cells, within inflammatory cells in airway lumina, and in alveolar walls. Other microbiological observations suggested that the ability of M. bovis to invade and cause lung parenchymal damage could be influenced by the participation of other pathogens.
对自然感染和实验感染牛支原体的犊牛肺组织进行组织病理学和免疫组织化学检查。免疫组织化学检查采用针对牛支原体的单克隆抗体和抗生物素蛋白-生物素-过氧化物酶复合物（ABC）检测底物系统。自然感染犊牛的肺部病变包括渗出性支气管肺炎和被炎性细胞包围的广泛凝固性坏死灶。实验感染的肺表现为化脓性细支气管炎和不同程度的支气管周围单核细胞套袖状浸润。在实际病例中，牛支原体抗原主要在凝固性坏死区域的周边、坏死渗出物中检测到，并与浸润的巨噬细胞和中性粒细胞密切相关。在诱发牛支原体肺炎的犊牛肺组织中，抗原位于上皮细胞、气道腔内的炎性细胞内以及肺泡壁中。其他微生物学观察结果表明，牛支原体侵入并导致肺实质损伤的能力可能受其他病原体参与情况的影响。
