Zhao Meng, Che Yanan, Gao Yan, Zhang Xiangyang
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
Front Pharmacol. 2024 Sep 6;15:1431862. doi: 10.3389/fphar.2024.1431862. eCollection 2024.
Traditional Chinese medicine (TCM) is playing an increasingly important role in disease treatment due to the advantages of multi-target, multi-pathway mechanisms, low adverse reactions and cost-effectiveness. However, the complexity of TCM system poses challenges for research. In recent years, there has been a surge in the application of multi-omics integrated research to explore the active components and treatment mechanisms of TCM from various perspectives, which aids in advancing TCM's integration into clinical practice and holds immense importance in promoting modernization. In this review, we discuss the application of proteomics, metabolomics, and mass spectrometry imaging in the study of composition, quality evaluation, target identification, and mechanism of action of TCM based on existing literature. We focus on the workflows and applications of multi-omics based on mass spectrometry in the research of TCM. Additionally, potential research ideas for future exploration in TCM are outlined. Overall, we emphasize the advantages and prospects of multi-omics based on mass spectrometry in the study of the substance basis and mechanism of action of TCM. This synthesis of methodologies holds promise for enhancing our understanding of TCM and driving its further integration into contemporary medical practices.
由于具有多靶点、多途径作用机制、不良反应少及成本效益高等优势，传统中药（TCM）在疾病治疗中发挥着越来越重要的作用。然而，中药体系的复杂性给研究带来了挑战。近年来，多组学整合研究的应用激增，旨在从多个角度探索中药的活性成分和治疗机制，这有助于推动中药融入临床实践，对促进其现代化具有重要意义。在本综述中，我们基于现有文献，探讨蛋白质组学、代谢组学和质谱成像在中药成分研究、质量评价、靶点识别及作用机制研究中的应用。我们重点关注基于质谱的多组学工作流程及其在中药研究中的应用。此外，还概述了未来中药研究的潜在思路。总体而言，我们强调基于质谱的多组学在中药物质基础和作用机制研究中的优势与前景。这种方法的综合有望加深我们对中药的理解，并推动其进一步融入当代医疗实践。
