Yu Dohyeon, Noh Dongho, Park Jinho
College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757 (Yu), and Chonbuk National University, Jeonju, 561-756 (Noh, Park), Korea.
Can J Vet Res. 2015 Jan;79(1):52-7.
Sepsis is associated with substantial morbidity and mortality in dogs. Alterations in hemostasis by systemic inflammation play an important role in the pathophysiology of sepsis. To evaluate the functional hemostatic changes in sepsis, we evaluated coagulation profiles and flow cytometric measurement of P-selectin (CD62P) expression on platelets, as well as platelet-leukocyte aggregation from a lipopolysaccharide (LPS)-induced endotoxemia model in dogs (n = 7). A sublethal dose of LPS [1 mg/kg body weight (BW)] induced thrombocytopenia and increased activated partial thromboplastin time (aPTT), prothrombin time (PT), and D-dimer concentrations. Flow cytometry analysis showed a significant increase in P-selectin expression on platelets between 1 and 24 h of a total 48 h of the experiment. In addition, platelet-leukocyte aggregation was significantly increased in the early stage of endotoxemia (at 1 and < 6 h for platelet-monocyte aggregation and at 3 h for platelet-neutrophil aggregation). Our results suggest that CD62P expression on platelets and platelet-leukocyte aggregation, as measured by flow cytometry, can be useful biomarkers of disseminated intravascular coagulation (DIC) in canine sepsis. These functional changes contribute to our understanding of the pathophysiology of hemostasis in endotoxemia.
脓毒症与犬类的高发病率和死亡率相关。全身炎症引起的止血改变在脓毒症的病理生理学中起重要作用。为了评估脓毒症中功能性止血变化，我们在犬类（n = 7）的脂多糖（LPS）诱导的内毒素血症模型中，评估了凝血谱、血小板上P-选择素（CD62P）表达的流式细胞术测量以及血小板-白细胞聚集情况。亚致死剂量的LPS [1 mg/kg体重（BW）] 导致血小板减少，并增加了活化部分凝血活酶时间（aPTT）、凝血酶原时间（PT）和D-二聚体浓度。流式细胞术分析显示，在总共48小时的实验中，1至24小时内血小板上P-选择素的表达显著增加。此外，在内毒素血症早期（血小板-单核细胞聚集在1小时和<6小时，血小板-中性粒细胞聚集在3小时），血小板-白细胞聚集显著增加。我们的结果表明，通过流式细胞术测量的血小板上CD62P的表达以及血小板-白细胞聚集，可作为犬类脓毒症中弥散性血管内凝血（DIC）的有用生物标志物。这些功能变化有助于我们理解内毒素血症中止血的病理生理学。
