Żmigrodzka Magdalena, Witkowska-Piłaszewicz Olga, Rzepecka Alicja, Cywińska Anna, Jagielski Dariusz, Winnicka Anna
Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (WULS-SGGW), Nowoursynowska 159c, 02-787 Warsaw, Poland.
Veterinary Clinic BIALOBRZESKA, Częstochowska 20, 02-344 Warsaw, Poland.
Animals (Basel). 2019 Aug 19;9(8):575. doi: 10.3390/ani9080575.
Extracellular vesicles (EVs) are a heterogeneous population of submicron-sized structures released during the activation, proliferation, or apoptosis of various types of cells. Due to their size, their role in cell-to-cell communication in cancer is currently being discussed. In blood, the most abundant population of EVs is platelet-derived EVs (PEVs). The aim of this study was to estimate the absolute number and the origin of EVs in the blood of healthy dogs and of dogs with various types of cancer. The EV absolute number and cellular origin were examined by flow cytometry technique. EVs were classified on the basis of surface annexin V expression (phosphatidylserine PS+) and co-expression of specific cellular markers (CD61, CD45, CD3, CD21). The number of PEVs was significantly higher in dogs with cancer (median: 409/µL, range: 42-2748/µL vs. median: 170/µL, range: 101-449/µL in controls). The numbers of EVs derived from leukocytes (control median: 86/µL, range: 40-240/µL; cancer median: 443/µL, range: 44-3 352/µL) and T cells (control median: 5/µL, range: 2-66/µL; cancer median: 108/µL, range: 3-1735/µL) were higher in dogs with neoplasia compared to healthy controls. The estimation of PEV and leukocyte-derived EV counts may provide a useful biological marker in dogs with cancer.
细胞外囊泡（EVs）是在各种类型细胞的激活、增殖或凋亡过程中释放的亚微米级结构的异质群体。由于其大小，目前正在讨论它们在癌症细胞间通讯中的作用。在血液中，最丰富的EVs群体是血小板衍生的EVs（PEVs）。本研究的目的是估计健康犬和患有各种类型癌症的犬血液中EVs的绝对数量和来源。通过流式细胞术技术检测EVs的绝对数量和细胞来源。根据表面膜联蛋白V表达（磷脂酰丝氨酸PS+）和特定细胞标志物（CD61、CD45、CD3、CD21）的共表达对EVs进行分类。患有癌症的犬的PEVs数量显著更高（中位数：409/μL，范围：42 - 2748/μL；而对照组中位数：170/μL，范围：101 - 449/μL）。与健康对照组相比，患有肿瘤的犬中来自白细胞的EVs数量（对照组中位数：86/μL，范围：40 - 240/μL；癌症组中位数：443/μL，范围：44 - 335μL）和T细胞来源的EVs数量（对照组中位数：5/μL，范围：2 - 66/μL；癌症组中位数：108/μL，范围：3 - 1735/μL）更高。PEV和白细胞衍生的EV计数的估计可能为患有癌症的犬提供一种有用的生物学标志物。
