长链非编码RNA CARLo-5的表达增强与胃癌的发生发展相关。
Enhanced expression of long noncoding RNA CARLo-5 is associated with the development of gastric cancer.
作者信息
Zhang Yan, Ma Mingzhe, Liu Weiyong, Ding Wenping, Yu Honggang
机构信息
Department of Gastroenterology, Renmin Hospital of Wuhan University Wuhan, Hubei, China ; Department of Gastroenterology, Yijishan Hospital Affiliated to Wannan Medical College Wuhu, Anhui, China.
Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
出版信息
Int J Clin Exp Pathol. 2014 Dec 1;7(12):8471-9. eCollection 2014.
The identification of cancer-associated long non-coding RNAs and the investigation of their molecular and biological functions are vital for understanding the molecular biology and progression of cancer. The CARLo-5, a newly identified long non-coding RNA, was found to be upregulated in colon cancer. However, little is known about its role in gastric cancer. In the present study, a great upregulation of CARLo-5 was observed in gastric cancer compared to paired adjacent normal tissues. Knockdown of CARLo-5 in gastric cancer cell lines significantly inhibited the cell proliferation via inducing G0/G1 cell-cycle arrest and apoptosis. Furthermore, ERK/MAPK pathway was found to be inactivated in the gastric cells after CARLo-5 knockdown. These results indicated that CARLo-5 might serve as a pro-oncogenic lncRNA that promotes proliferation of gastric cancer and activates the ERK/MAPK pathway.
鉴定癌症相关的长链非编码RNA并研究其分子和生物学功能对于理解癌症的分子生物学和进展至关重要。CARLo-5是一种新鉴定出的长链非编码RNA，在结肠癌中被发现上调。然而，其在胃癌中的作用知之甚少。在本研究中，与配对的相邻正常组织相比，在胃癌中观察到CARLo-5显著上调。在胃癌细胞系中敲低CARLo-5通过诱导G0/G1期细胞周期停滞和凋亡显著抑制细胞增殖。此外，发现敲低CARLo-5后胃癌细胞中的ERK/MAPK通路失活。这些结果表明，CARLo-5可能作为一种促癌lncRNA促进胃癌增殖并激活ERK/MAPK通路。
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