霍乱弧菌O1生物型E1 Tor菌毛：黏附性菌株与非黏附性菌株的比较研究

Pili of Vibrio cholerae O1 biotype E1 Tor: a comparative study on adhesive and non-adhesive strains.

作者信息

Iwanaga M, Nakasone N, Ehara M

机构信息

Department of Bacteriology, University of Ryukyus School of Medicine, Okinawa.

出版信息

Microbiol Immunol. 1989;33(1):1-9. doi: 10.1111/j.1348-0421.1989.tb01492.x.

DOI:10.1111/j.1348-0421.1989.tb01492.x

PMID:2567485

Abstract

Pili were found on the cell surface of non-adhesive Vibrio cholerae O1 Biotype E1 Tor as well as the adhesive strain. Purified pili of the adhesive and non-adhesive strains were morphologically, electrophoretically, and immunologically, indistinguishable from each other. The molecular weights of both pilin (subunit protein of the pilus) were about 16,000 daltons as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These 16 kDa pili are different from the pilus colonization factor, which is a 20.5 kDa protein, reported by Taylor et al. The 16 kDa pili of Vibrio cholerae O1 Biotype E1 Tor have hemagglutinating activity, but may have no role in colonization, because non-adhesive strains also have such pili.

摘要

在非黏附性霍乱弧菌O1生物型E1 Tor以及黏附性菌株的细胞表面发现了菌毛。黏附性菌株和非黏附性菌株的纯化菌毛在形态学、电泳和免疫学方面彼此无法区分。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，两种菌毛蛋白（菌毛的亚基蛋白）的分子量均约为16,000道尔顿。这些16 kDa的菌毛不同于Taylor等人报道的作为20.5 kDa蛋白的菌毛定植因子。霍乱弧菌O1生物型E1 Tor的16 kDa菌毛具有血凝活性，但可能在定植过程中不起作用，因为非黏附性菌株也有这种菌毛。