霍乱弧菌O139菌毛的纯化与特性分析

Purification and characterization of vibrio cholerae O139 fimbriae.

作者信息

Yamashiro T, Nakasone N, Honma Y, Albert M J, Iwanaga M

机构信息

Department of Bacteriology, University of the Ryukyus, School of Medicine, Okinawa, Japan.

出版信息

FEMS Microbiol Lett. 1994 Jan 15;115(2-3):247-52. doi: 10.1111/j.1574-6968.1994.tb06646.x.

DOI:10.1111/j.1574-6968.1994.tb06646.x

PMID:7908003

Abstract

A Vibrio cholerae O139 (strain Al-1841) isolated from a patient with a cholera-like disease in Bangladesh predominantly produced new curved, wavy fimbriae (Al-1841 fimbriae) and small numbers of previously reported V. cholerae non-O1 S7-like pili. The former was purified and characterized. The molecular mass of the Al-1841 fimbrial subunit was less than 2.5 kDa, and it was immunologically different from that of V. cholerae non-O1 S7 pili. This novel fimbrial antigen was detected in all 182 Gram-negative strains from five genera tested but was absent from the Gram-positive bacteria tested. The purified Al-1841 fimbriae did not agglutinate human or rabbit erythrocytes.

摘要

从孟加拉国一名霍乱样疾病患者身上分离出的一株霍乱弧菌O139（菌株Al-1841）主要产生新的弯曲、波浪状菌毛（Al-1841菌毛）以及少量先前报道的霍乱弧菌非O1 S7样菌毛。对前者进行了纯化和特性鉴定。Al-1841菌毛亚基的分子量小于2.5 kDa，并且在免疫方面与霍乱弧菌非O1 S7菌毛不同。在测试的来自五个属的所有182株革兰氏阴性菌株中均检测到这种新型菌毛抗原，但在测试的革兰氏阳性细菌中未检测到。纯化后的Al-1841菌毛不会凝集人或兔红细胞。