霍乱弧菌菌株菌毛的纯化与特性分析：一种可能的定植因子
Purification and characterization of a pilus of a Vibrio cholerae strain: a possible colonization factor.
作者信息
Yamashiro T, Iwanaga M
机构信息
Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
出版信息
Infect Immun. 1996 Dec;64(12):5233-8. doi: 10.1128/iai.64.12.5233-5238.1996.
A new flexible type of pilus was purified from Vibrio cholerae non-O1, non-0139 strain NAGV14 and characterized. The molecular mass of the pilin was estimated to be 20 kDa, and the antigenicity differed from that of known pili such as toxin-coregulated pili, mannose-sensitive hemagglutinating pili, V10 pili, and Al-1841 pili. The NAGV14 pilus was regarded as a colonization factor because the purified pili adhered to rabbit intestine and adhesion was inhibited by treating the organisms with the Fab fraction of an antipilus antibody. An intestinal receptor blockade using purified pili failed to inhibit adhesion of the organisms. The NAGV14 pili adhered to the surface of live V. cholerae. An antigen cross-reacting with the NAGV14 pili was widely and specifically distributed among V. cholerae strains irrespective of serotype and biotype. The amino acid sequence of the pilin was homologous with that of MshA. The NAGV14 pili did not agglutinate human and rabbit erythrocytes.
从霍乱弧菌非O1、非O139菌株NAGV14中纯化出一种新型柔性菌毛并对其进行了表征。菌毛蛋白的分子量估计为20 kDa，其抗原性与已知菌毛不同，如毒素共调节菌毛、甘露糖敏感血凝菌毛、V10菌毛和Al-1841菌毛。NAGV14菌毛被视为一种定植因子，因为纯化的菌毛可黏附于兔肠道，且用抗菌毛抗体的Fab片段处理细菌后黏附受到抑制。使用纯化菌毛进行肠道受体阻断未能抑制细菌的黏附。NAGV14菌毛黏附于活的霍乱弧菌表面。与NAGV14菌毛发生交叉反应的抗原广泛且特异性地分布于霍乱弧菌菌株中，与血清型和生物型无关。菌毛蛋白的氨基酸序列与MshA的氨基酸序列同源。NAGV14菌毛不凝集人和兔的红细胞。
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