Iwanaga M, Nakasone N, Yamashiro T, Higa N
Department of Bacteriology, University of the Ryukyus School of Medicine, Okinawa, Japan.
Microbiol Immunol. 1993;37(1):23-8. doi: 10.1111/j.1348-0421.1993.tb03174.x.
The distribution of Vibrio cholerae O1 pili consisting of 16 kDa subunit protein (16K-pili) was examined by Western blotting, using 211 strains from various origins and specific anti-16K-pili sera. The 16 kDa protein was detected in all 211 strains. The pili were purified from 3 El Tor and 3 classical strains, and characterized by hemagglutination and inhibition tests. All purified pili were hemagglutinative. However, the hemagglutinating activity of classical pili disappeared after exposure to 5 M urea and the agglutination induced by the classical pili was inhibited by D-mannose, alpha-methylmannoside, D-glucose and N-acetylglucosamine. On the contrary, El Tor pili were resistant to these sugars and urea.
利用来自不同来源的211株菌株和特异性抗16K菌毛血清，通过蛋白质免疫印迹法检测了由16 kDa亚基蛋白组成的霍乱弧菌O1菌毛（16K菌毛）的分布情况。在所有211株菌株中均检测到了16 kDa蛋白。从3株埃尔托生物型菌株和3株古典生物型菌株中纯化出菌毛，并通过血凝试验和抑制试验对其进行了鉴定。所有纯化的菌毛都具有血凝活性。然而，古典生物型菌毛在暴露于5 M尿素后血凝活性消失，且由古典生物型菌毛诱导的凝集反应受到D-甘露糖、α-甲基甘露糖苷、D-葡萄糖和N-乙酰葡糖胺的抑制。相反，埃尔托生物型菌毛对这些糖类和尿素具有抗性。
