来自裂榄树的羽扇烯衍生五环三萜对单纯疱疹病毒1型和2型体外复制的抑制作用
Inhibitory effects of lupene-derived pentacyclic triterpenoids from Bursera simaruba on HSV-1 and HSV-2 in vitro replication.
作者信息
Álvarez Ángel L, Habtemariam Solomon, Parra Francisco
机构信息
a Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo , Oviedo , 33006 Asturias , Spain.
b Pharmacognosy Research Laboratories, Medway School of Science, University of Greenwich , Central Avenue, Chatham-Maritime, Kent ME4 4TB , UK.
出版信息
Nat Prod Res. 2015;29(24):2322-7. doi: 10.1080/14786419.2015.1007456. Epub 2015 Feb 12.
The cytotoxicity and antiviral properties of Bursera simaruba against herpes simplex viruses (HSV-1 and HSV-2) were investigated through a bioactivity-guided isolation protocol. The plant material was fractionated using solvent-solvent partitioning, size-exclusion and thin-layer chromatography. The antiviral compounds present in the most active fractions were identified by means of LC-MS and NMR. Three different methods were compared during the evaluation of antiviral activity of samples. Four lupene-related pentacyclic triterpenes were found to be responsible for the anti-herpesvirus effects of B. simaruba and were isolated from this species for the first time. The selective indexes (SI) of B. simaruba-derived samples ranged from 7.7 to 201.9.
通过生物活性导向分离方案研究了裂榄（Bursera simaruba）对单纯疱疹病毒（HSV - 1和HSV - 2）的细胞毒性和抗病毒特性。使用溶剂 - 溶剂分配、尺寸排阻和薄层色谱对植物材料进行分级分离。通过液相色谱 - 质谱联用（LC - MS）和核磁共振（NMR）鉴定最具活性级分中存在的抗病毒化合物。在评估样品的抗病毒活性期间比较了三种不同方法。发现四种与羽扇烯相关的五环三萜是裂榄抗疱疹病毒作用的原因，并且首次从该物种中分离出来。裂榄衍生样品的选择性指数（SI）范围为7.7至201.9。
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