Toplu N, Oğuzoğlu T Ç, Ural K, Albayrak H, Ozan E, Ertürk A, Epikmen E T
Department of Pathology, Adnan Menderes University, Aydin, Turkey
Department of Virology, Ankara University, Ankara, Turkey.
Vet Pathol. 2015 Nov;52(6):1073-6. doi: 10.1177/0300985815570067. Epub 2015 Feb 12.
This study describes the clinicopathologic findings in naturally occurring West Nile virus (WNV) infection in horses. WNV was diagnosed in a foal by immunohistochemical and in situ hybridization methods, and the presence of WNV antibodies was detected in 5 other horses with clinical signs suggestive of WNV infection. At necropsy of the foal, lymph nodes were edematous and enlarged, and the intestines showed diffuse congestion and focal hemorrhages. The most significant histologic lesions in this case were nonsuppurative meningoencephalomyelitis, particularly in the brainstem and spinal cord. Identification of viral RNA by in situ hybridization and viral antigen by immunohistochemistry was concentrated primarily in nerve fibers, glial cells, and their processes in brainstem and spinal cord and, to a lesser extent, within the cerebral hemispheres and cerebellum.
本研究描述了马自然感染西尼罗河病毒（WNV）的临床病理特征。通过免疫组织化学和原位杂交方法在一匹幼驹中诊断出WNV感染，并且在另外5匹有提示WNV感染临床症状的马中检测到了WNV抗体。对该幼驹进行尸检时，淋巴结水肿且肿大，肠道呈现弥漫性充血和局灶性出血。该病例中最显著的组织学病变是非化脓性脑膜脑脊髓炎，尤其是在脑干和脊髓。通过原位杂交鉴定病毒RNA以及通过免疫组织化学鉴定病毒抗原主要集中在脑干和脊髓的神经纤维、神经胶质细胞及其突起中，在大脑半球和小脑中的程度较轻。
