马蹄蝠中RIG-I、STAT-1和IFN-β的分子特征分析
Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat.
作者信息
Li Jinju, Zhang Guangxu, Cheng Dalong, Ren Hua, Qian Min, Du Bing
机构信息
Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.
Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.
出版信息
Gene. 2015 Apr 25;561(1):115-23. doi: 10.1016/j.gene.2015.02.013. Epub 2015 Feb 10.
Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection.
野生中华菊头蝠已被证明是类严重急性呼吸综合征冠状病毒的天然宿主。然而，蝙蝠关键蛋白的分子特征仍有待进一步探索。在本研究中，我们利用克隆和生物信息学方法分析了来自中菊头蝠和中华菊头蝠永生化细胞系中视黄酸诱导基因I（RIG-I）、信号转导和转录激活因子1（STAT-1）以及干扰素-β（IFN-β）的序列。然后，我们用聚肌苷酸-聚胞苷酸（polyI:C）和水疱性口炎病毒（VSV）处理不同的蝙蝠细胞、小鼠胚胎成纤维细胞（MEF）和脾细胞，以评估和比较蝙蝠与小鼠之间的抗病毒免疫反应。我们的结果表明，蝙蝠的RIG-I、STAT-1和IFN-β与人类、小鼠、猪和恒河猴具有高度同源性。RIG-I和STAT-1在蝙蝠脾脏中均高表达。此外，polyI:C和VSV在蝙蝠和小鼠细胞中均能诱导IFN-β产生。这些发现为蝙蝠针对病毒感染的固有免疫系统的潜在特征提供了新的见解。
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