Liang Ying-Zi, Wu Li-Jun, Zhang Qian, Zhou Peng, Wang Mei-Niang, Yang Xing-Lou, Ge Xing-Yi, Wang Lin-Fa, Shi Zheng-Li
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore, 169857, Singapore.
Virol Sin. 2015 Dec;30(6):425-32. doi: 10.1007/s12250-015-3668-2. Epub 2015 Dec 7.
Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats. Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.
蝙蝠是许多病毒的天然宿主，这些病毒在蝙蝠体内不会产生临床症状。因此，蝙蝠可能已经进化出有效的机制来控制病毒复制。然而，关于蝙蝠对病毒感染的免疫反应的信息却很少。I型干扰素（IFN）在控制病毒感染中起关键作用。在本研究中，我们报道了来自中国小蝙蝠大足鼠耳蝠的干扰素β（IFNβ）的克隆、表达及生物学活性。我们证明了在用聚肌胞苷酸（polyI:C）处理或感染仙台病毒后，大足鼠耳蝠来源的肾细胞系中IFNB和IFN刺激基因的上调。此外，重组IFNβ抑制了来自两种蝙蝠物种——大足鼠耳蝠和中华菊头蝠的细胞系中的水疱性口炎病毒和蝙蝠腺病毒的复制。我们提供了小蝙蝠中IFNβ抗病毒活性的首个体外证据，这对于病毒与这些宿主的相互作用具有重要意义。
