Araújo B C, Moraes M S, Costa L E O, Nascimento J S
Laboratory of Microbiology, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rua Senador Furtado 121, Maracanã, Rio de Janeiro, CEP 20270-021, Brazil.
Laboratory of Microbiology, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rua Senador Furtado 121, Maracanã, Rio de Janeiro, CEP 20270-021, Brazil.
J Dairy Sci. 2015 Apr;98(4):2303-6. doi: 10.3168/jds.2014-8825. Epub 2015 Feb 11.
Infant milk formulas are not sterile products, and pathogenic bacteria can survive and multiply in these products. This study was performed, initially, to detect the presence of Salmonella spp. in reconstituted infant milk formula and on utensils previously sanitized used in their preparation or distribution in a nursery of a public hospital in Rio de Janeiro. None of the samples tested carried Salmonellaspp. However, further identification of colonies growing on the selective media revealed the presence of several other gram-negative bacteria. Seventeen isolates were identified as belonging to Acinetobacter baumannii-calcoaceticus complex. Fourteen isolates presented a multidrug-resistance profile, by disc diffusion assays, and one of them--JE4--was also resistant to imipenem. The detection of Acinetobacter isolates in this work demonstrates inadequate hygiene practices in the preparation or distribution of infant milk formula.
婴儿配方奶粉并非无菌产品，病原菌可在这些产品中存活并繁殖。本研究最初旨在检测里约热内卢一家公立医院托儿所中用于调配或分发婴儿配方奶粉的已复原奶粉及先前消毒过的器具上是否存在沙门氏菌属。所检测的样本均未携带沙门氏菌属。然而，对在选择性培养基上生长的菌落进行进一步鉴定后发现存在几种其他革兰氏阴性菌。17株分离菌被鉴定为鲍曼不动杆菌-醋酸钙不动杆菌复合体。通过纸片扩散法检测，14株分离菌呈现多重耐药谱，其中一株——JE4——还对亚胺培南耐药。在这项研究中检测到不动杆菌分离菌表明在婴儿配方奶粉的调配或分发过程中存在卫生措施不当情况。
