Keller G, Wagner E F
Basel Institute for Immunology, Switzerland.
Genes Dev. 1989 Jun;3(6):827-37. doi: 10.1101/gad.3.6.827.
A recombinant retrovirus, N-TK-src, was used to introduce the v-src oncogene into mouse hematopoietic cells. This vector efficiently expresses both the neo and v-src genes in different hematopoietic lineages in culture as well as in mice reconstituted with infected bone marrow cells. Expression of v-src had no dramatic effect on the proliferative and differentiative capacity of hematopoietic precursors when assayed in methyl cellulose cultures. However, in mice reconstituted with N-TK-src-infected bone marrow cells, expression of v-src leads to the rapid development of a severe myeloproliferative disease, characterized by splenomegaly, anemia, and a shift of hematopoiesis from the bone marrow to the spleen.
一种重组逆转录病毒N-TK-src被用于将v-src癌基因导入小鼠造血细胞。该载体在培养的不同造血谱系以及用感染的骨髓细胞重建的小鼠中均能有效表达新霉素基因和v-src基因。当在甲基纤维素培养物中检测时，v-src的表达对造血前体细胞的增殖和分化能力没有显著影响。然而，在用N-TK-src感染的骨髓细胞重建的小鼠中，v-src的表达导致严重骨髓增殖性疾病的快速发展，其特征为脾肿大、贫血以及造血从骨髓转移至脾脏。
