International Center for Tropical Agriculture, P.O. Box 6247, Kampala, Uganda.
Rwanda Agriculture Board, P.O. Box 5016, Kigali, Rwanda.
FEMS Microbiol Lett. 2015 Feb;362(4). doi: 10.1093/femsle/fnu055. Epub 2014 Dec 11.
We announce the genome sequence for Xanthomonas species strain Nyagatare, isolated from beans showing unusual disease symptoms in Rwanda. This strain represents the first sequenced genome belonging to an as-yet undescribed Xanthomonas species known as species-level clade 1. It has at least 100 kb of genomic sequence that shows little or no sequence similarity to other xanthomonads, including a unique lipopolysaccharide synthesis gene cluster. At least one genomic region appears to have been acquired from relatives of Agrobacterium or Rhizobium species. The genome encodes homologues of only three known type-three secretion system effectors: AvrBs2, XopF1 and AvrXv4. Availability of the genome sequence will facilitate development of molecular tools for detection and diagnostics for this newly discovered pathogen of beans and facilitate epidemiological investigations of a potential causal link between this pathogen and the disease outbreak.
我们宣布，从卢旺达表现出异常疾病症状的豆类中分离到的黄单胞菌菌株 Nyagatare 的基因组序列。该菌株代表了第一个被测序的属于尚未被描述的黄单胞菌物种的基因组，该物种被称为种级 1 类群。它至少有 100 kb 的基因组序列与其他黄单胞菌显示出很少或没有序列相似性，包括一个独特的脂多糖合成基因簇。至少有一个基因组区域似乎是从根瘤菌或土壤杆菌属的亲缘种中获得的。基因组仅编码三个已知的 III 型分泌系统效应物的同源物：AvrBs2、XopF1 和 AvrXv4。该基因组序列的可用性将有助于开发针对这种新发现的豆类病原体的分子检测和诊断工具，并有助于对这种病原体与疾病爆发之间的潜在因果关系进行流行病学调查。
