IRD, Cirad, Université Montpellier, IPME, Montpellier, France.
Nature Research Centre, Institute of Botany, Laboratory of Plant Pathology, Akademijos g. 2, Vilnius, Lithuania.
Phytopathology. 2021 Apr;111(4):611-616. doi: 10.1094/PHYTO-07-20-0273-SC. Epub 2021 Feb 26.
is the causal agent of bacterial canker on tea plants. There is no complete genome sequence available for , a close relative of the species and , thus limiting basic research for this group of pathogens. Here, we release a high-quality complete genome sequence for the type strain, CFBP 4691. Single-molecule real-time sequencing with a mean coverage of 264× revealed two contigs of 4,744,641 bp (chromosome) and 40,955 bp (plasmid) in size. Genome mining revealed the presence of nonribosomal peptide synthases, two CRISPR systems, the Xps type 2 secretion system, and the Hrp type 3 secretion system. Surprisingly, this strain encodes an additional type 2 secretion system and a novel type 3 secretion system with enigmatic function, hitherto undescribed for xanthomonads. Four type 3 effector genes were found on complete or partial transposons, suggesting a role of transposons in effector gene evolution and spread. This genome sequence fills an important gap to better understand the biology and evolution of the early-branching xanthomonads, also known as clade-1 xanthomonads.
是茶树细菌性溃疡病的病原体。由于缺乏其近缘种 和 的完整基因组序列，限制了对这组病原菌的基础研究。在这里，我们发布了 型菌株 CFBP 4691 的高质量完整基因组序列。单分子实时测序的平均覆盖率为 264×，结果显示该菌株的大小分别为 4744641 bp（染色体）和 40955 bp（质粒）的两个连续序列。基因组挖掘揭示了非核糖体肽合成酶、两个 CRISPR 系统、Xps 型 2 型分泌系统和 Hrp 型 3 型分泌系统的存在。令人惊讶的是，该菌株还编码了一个额外的 2 型分泌系统和一个具有神秘功能的新型 3 型分泌系统，这在黄单胞菌中是前所未有的。四个 3 型效应基因存在于完整或部分转座子上，这表明转座子在效应基因进化和传播中发挥了作用。该基因组序列填补了一个重要的空白，有助于更好地理解早期分支的黄单胞菌（也称为 1 型黄单胞菌）的生物学和进化。
