Arends Fabienna, Nowald Constantin, Pflieger Kerstin, Boettcher Kathrin, Zahler Stefan, Lieleg Oliver
Institute of Medical Engineering IMETUM, Technische Universität München, Boltzmannstrasse 11, 85748, Garching, Germany; Department of Mechanical Engineering, Technische Universität München, Boltzmannstrasse 15, 85748, Garching, Germany.
Department of Pharmacy-Center for Drug Research, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, 81377, München, Germany.
PLoS One. 2015 Feb 17;10(2):e0118090. doi: 10.1371/journal.pone.0118090. eCollection 2015.
The migration of cells within a three-dimensional extracellular matrix (ECM) depends sensitively on the biochemical and biophysical properties of the matrix. An example for a biological ECM is given by reconstituted basal lamina gels purified from the Engelbreth-Holm-Swarm sarcoma of mice. Here, we compare four different commercial variants of this ECM, which have all been purified according to the same protocol. Nevertheless, in those gels, we detect strong differences in the migration behavior of leukocyte cells as well as in the Brownian motion of nanoparticles. We show that these differences correlate with the mechanical properties and the microarchitecture of the gels which in turn arise from small variations in their biochemical composition.
细胞在三维细胞外基质（ECM）中的迁移对基质的生化和生物物理特性敏感。从小鼠的恩格尔布雷特-霍尔姆-斯旺肉瘤中纯化得到的重组基膜凝胶就是一种生物ECM的例子。在此，我们比较了这种ECM的四种不同商业变体，它们均按照相同的方案进行纯化。然而，在这些凝胶中，我们检测到白细胞迁移行为以及纳米颗粒布朗运动存在显著差异。我们表明，这些差异与凝胶的机械性能和微观结构相关，而凝胶的机械性能和微观结构又源于其生化组成的微小变化。
