Singh Priyanka, Kim Yeon-Ju, Nguyen Ngoc-Lan, Hoang Van-An, Sukweenadhi Johan, Farh Mohamed El-Agamy, Yang Deok-Chun
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Gyeonggi-do, Yongin-si, 446-701, Republic of Korea.
Antonie Van Leeuwenhoek. 2015 Mar;107(3):749-58. doi: 10.1007/s10482-014-0369-z. Epub 2015 Feb 19.
A novel bacterial strain, DCY86(T) (=KCTC 42053(T) = JCM 19890(T)) was isolated from soil of a ginseng field in Yeoncheon province (38°04'00″N 126°57'00″E), Republic of Korea using a serial dilution method. Strain DCY86(T) was observed to be Gram-stain negative, strictly aerobic, to grow optimally at 25-30 °C, at pH 7-7.5 and on tryptic soya agar medium. The cells were found to be sensitive to ceftazidine and tetracycline. Based on 16S rRNA gene sequence comparisons, strain DCY86(T) was found to be most closely related to Cupriavidus basilensis LMG 18990(T) (98.48 %), Cupriavidus numazensis LMG 26411(T) (98.34 %), Cupriavidus pinatabonesis KCTC 22125(T) (98.34 %) and Cupriavidus laharis KCTC 22126(T) (98.00 %). The G+C content was determined to be 64.23 mol %. The only isoprenoid quinone detected in strain DCY86(T) was ubiquinone Q-8. The major polar lipids were identified as diphosphatidylglycerol, phosphtidylethanolamine, phosphatidylglycerol, unidentified aminophosphoglycolipids and unidentified phospholipids. The major fatty acids were identified as C16:0 summed feature 3 (C16:1 ω7c/ω6c and/or iso-C15 : 0 2-OH) and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). These data support the affiliation of strain DCY86(T) to the genus Cupriavidus. Strain DCY86(T) was also found to be able to solubilize phosphate and produce siderophores. The results of physiological and biochemical tests enabled strain DCY86(T) to be differentiated genotypically and phenotypically from the recognized species of the genus Cupriaividus. Therefore, the novel isolate can be considered to represent a novel species, for which the name Cupriavidus yeoncheonense sp. nov. is proposed here. The type strain is DCY86(T) (=KCTC 42053(T) = JCM 19890(T)).
采用连续稀释法从韩国涟川省（北纬38°04'00″，东经126°57'00″）人参田土壤中分离出一株新型细菌菌株DCY86(T)（=KCTC 42053(T)=JCM 19890(T)）。观察到菌株DCY86(T)革兰氏染色阴性，严格需氧，在25-30°C、pH 7-7.5条件下以及胰蛋白胨大豆琼脂培养基上生长最佳。发现该细胞对头孢他啶和四环素敏感。基于16S rRNA基因序列比较，发现菌株DCY86(T)与巴西贪铜菌LMG 18990(T)（98.48%）、沼津贪铜菌LMG 26411(T)（98.34%）、皮纳贪铜菌KCTC 22125(T)（98.34%）和拉哈贪铜菌KCTC 22126(T)（98.00%）关系最为密切。G+C含量测定为64.23 mol%。在菌株DCY86(T)中检测到的唯一类异戊二烯醌是泛醌Q-8。主要极性脂类被鉴定为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、未鉴定的氨基磷脂糖脂和未鉴定的磷脂。主要脂肪酸被鉴定为C16:0总和特征3（C16:1 ω7c/ω6c和/或异-C15:0 2-OH）和总和特征8（C18:1 ω7c和/或C18:1 ω6c）。这些数据支持菌株DCY86(T)属于贪铜菌属。还发现菌株DCY86(T)能够溶解磷酸盐并产生铁载体。生理生化测试结果使菌株DCY86(T)在基因型和表型上与贪铜菌属已确认的物种区分开来。因此，该新分离株可被认为代表一个新物种，在此提议将其命名为涟川贪铜菌（Cupriavidus yeoncheonense sp. nov.）。模式菌株为DCY86(T)（=KCTC 42053(T)=JCM 19890(T)）。
