Dirkzwager Roderick M, Kinghorn Andrew B, Richards Jack S, Tanner Julian A
Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R..
Chem Commun (Camb). 2015 Mar 18;51(22):4697-700. doi: 10.1039/c5cc00438a.
We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL(-1)) and could reliably diagnose malaria in clinical blood samples.
我们报告了通过适体连接酶捕获法（APTEC）对疟疾进行快速诊断的方法，即适体捕获生物标志物恶性疟原虫乳酸脱氢酶（PfLDH），然后通过比色法测量其活性。该稳健的检测方法灵敏度高（检测限 = 4.9 ng mL(-1)），能够可靠地诊断临床血液样本中的疟疾。
