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使用基于微流控的恶性疟原虫乳酸脱氢酶免疫测定法的简单、快速且准确的疟疾诊断平台。

Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase.

作者信息

Lee Wang Sik, Kang Taejoon, Kwak Kyung Jin, Park Kyoungsook, Yi So Yeon, Lee Ui Jin, Shin Yong Beom, Jeong Jinyoung

机构信息

Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea.

出版信息

Nano Converg. 2020 Apr 11;7(1):13. doi: 10.1186/s40580-020-00223-w.

DOI:10.1186/s40580-020-00223-w
PMID:32279129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7150666/
Abstract

This work reports on a rapid diagnostic platform for the detection of Plasmodium falciparum lactate dehydrogenase (PfLDH), a representative malaria biomarker, using a microfluidic microplate-based immunoassay. In this study, the microfluidic microplate made it possible to diagnose PfLDH with a small volume of sample (only 5 μL) and short time (< 90 min) compared to conventional immunoassays such as enzyme-linked immunosorbent assay (ELISA). Moreover, the diagnostic performance of PfLDH showed high sensitivity, specificity, and selectivity (i.e., 0.025 pg/μL in phosphate-buffered saline and 1 pg/μL in human serum). The microfluidic-based microplate sensing platform has the potential to adapt simple, rapid, and accurate diagnoses to the practical detection of malaria.

摘要

这项工作报道了一种基于微流控微孔板免疫测定法的快速诊断平台，用于检测恶性疟原虫乳酸脱氢酶（PfLDH），这是一种具有代表性的疟疾生物标志物。在本研究中，与传统免疫测定法（如酶联免疫吸附测定法（ELISA））相比，微流控微孔板能够使用少量样本（仅5 μL）并在短时间内（< 90分钟）诊断PfLDH。此外，PfLDH的诊断性能表现出高灵敏度、特异性和选择性（即在磷酸盐缓冲盐水中为0.025 pg/μL，在人血清中为1 pg/μL）。基于微流控的微孔板传感平台有潜力将简单、快速且准确的诊断应用于疟疾的实际检测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/07de50a925ef/40580_2020_223_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/a3b223ab25ba/40580_2020_223_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/1722d18cea2f/40580_2020_223_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/6320ce3c9e5a/40580_2020_223_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/da2840736d2d/40580_2020_223_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/742c4c36c27a/40580_2020_223_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/07de50a925ef/40580_2020_223_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/a3b223ab25ba/40580_2020_223_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/1722d18cea2f/40580_2020_223_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/6320ce3c9e5a/40580_2020_223_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/da2840736d2d/40580_2020_223_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/742c4c36c27a/40580_2020_223_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/106f/7150666/07de50a925ef/40580_2020_223_Fig6_HTML.jpg

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