Wolf Tiffany M, Mugisha Lawrence, Shoyama Fernanda Miyagaki, O'Malley Melanie J, Flynn JoAnne L, Asiimwe Benon, Travis Dominic A, Singer Randall S, Sreevatsan Srinand
Emerg Infect Dis. 2015 Mar;21(3):468-70. doi: 10.3201/eid2103.140052.
Traditional testing methods have limited epidemiologic studies of tuberculosis among free-living primates. PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis from fecal samples was evaluated as a noninvasive screening test for tuberculosis in primates. Active tuberculosis was detected among inoculated macaques and naturally exposed chimpanzees, demonstrating the utility of this test.
传统检测方法限制了对自由生活灵长类动物结核病的流行病学研究。对粪便样本中结核分枝杆菌插入元件IS6110进行PCR扩增,被评估为灵长类动物结核病的一种非侵入性筛查检测方法。在接种的猕猴和自然暴露的黑猩猩中检测到活动性结核病,证明了该检测方法的实用性。