人类蛋白质组中不存在X染色体剂量补偿。
No X-chromosome dosage compensation in human proteomes.
作者信息
Chen Xiaoshu, Zhang Jianzhi
机构信息
Department of Ecology and Evolutionary Biology, University of Michigan.
Department of Ecology and Evolutionary Biology, University of Michigan
出版信息
Mol Biol Evol. 2015 Jun;32(6):1456-60. doi: 10.1093/molbev/msv036. Epub 2015 Feb 19.
Abstract
The X and Y chromosomes of placental and marsupial mammals originated from a pair of autosomes. Ohno proposed that the expression levels of X-linked genes must have been doubled in males to compensate for the degeneration of their Y homologs. Recent mRNA sequencing experiments, however, found at most weak or infrequent X-chromosome dosage compensation. Nonetheless, dosage compensation need not occur at the mRNA level, because ultimately it is the protein concentration that matters. Analyzing human proteomic data from 22 tissues, we here report that X upregulation is absent at the protein level, indicating that Ohno's hypothesis is also invalid at the protein level.
摘要
胎盘哺乳动物和有袋类哺乳动物的X和Y染色体起源于一对常染色体。大野干提出，雄性X连锁基因的表达水平必定加倍，以补偿其Y同源染色体的退化。然而，最近的mRNA测序实验发现，X染色体剂量补偿最多只是微弱或不常见的。尽管如此，剂量补偿不一定发生在mRNA水平，因为最终起关键作用的是蛋白质浓度。通过分析来自22个组织的人类蛋白质组数据，我们在此报告，在蛋白质水平上不存在X上调现象，这表明大野干的假设在蛋白质水平上也是不成立的。
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