使用MFEprimer选择特定的PCR引物。

Selecting specific PCR primers with MFEprimer.

作者信息

Qu Wubin, Zhang Chenggang

机构信息

Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center of PLA, Beijing, 100850, China.

出版信息

Methods Mol Biol. 2015;1275:201-13. doi: 10.1007/978-1-4939-2365-6_15.

DOI:10.1007/978-1-4939-2365-6_15

PMID:25697662

Abstract

Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA. In this chapter, we introduce: (1) the factors that affect the specificity of primers; (2) the principle of MFEprimer and its settings; (3) how to use the MFEprimer to examine the specificity of primers.

摘要

选择特定的引物对聚合酶链反应（PCR）至关重要。非特异性引物会与非预期基因结合，导致产生非特异性扩增子。MFEprimer是一个用于针对背景DNA检查PCR引物特异性的程序。在本章中，我们将介绍：（1）影响引物特异性的因素；（2）MFEprimer的原理及其设置；（3）如何使用MFEprimer来检测引物的特异性。