Odhoch Phenny Sharon, Budambula Nancy L M, Kiprotich Felix, Muli Joshua Kiilu
Department of Biological Sciences, University of Embu, Embu, Kenya.
Department of Biological Sciences, University of Kabianga, Kericho, Kenya.
Scientifica (Cairo). 2025 Jun 1;2025:2409286. doi: 10.1155/sci5/2409286. eCollection 2025.
is a plant genus with more than 700 species of shrubs and herbs. Despite its potential economic importance, has received limited research attention; hence, there is limited information on its genetic diversity. Hence, there is need to establish its genetic diversity as a foundation for its conservation and breeding. The current study aimed to optimize and validate simple sequence repeat (SSR) markers polymerase chain reaction-conditions for the assessment of genetic diversity in The genomic DNA of 31 accessions was extracted from 2-week-old leaves using a modified CTAB protocol and Quick-DNA Plant/Seed Kits (Zymo Research Corp) were used for recalcitrant samples. The samples were then amplified using the 29 SSR markers under the optimized conditions. The polymorphism information content (PIC) of the polymorphic markers was calculated to determine their effectiveness. This study determined that the optimal concentrations of dNTPs, MgCl, and primers as 2.5, 2, and 5 mM, respectively, and the quantity of the DNA template was 1 μL, and the quantity of was 0.125 μL in a 25 μL reaction mixture. The mean PIC value was 0.233, which shows that the markers were slightly informative. The marker PC004 was the most informative marker with the highest PIC value (0.605) and it detected the largest number of alleles despite being a hexanucleotide motif repeat. Its uniqueness augments its potential use in the assessment of genetic diversity. This study implies that the SSR markers designed and optimized for the study are significant for genetic diversity and population structure analysis of species and molecular verification of genotypes as well as other related genera. Besides, the results of the study form a basis for genetic improvement of
是一个拥有700多种灌木和草本植物的植物属。尽管其具有潜在的经济重要性，但受到的研究关注有限；因此，关于其遗传多样性的信息也很有限。因此，有必要确定其遗传多样性，为其保护和育种奠定基础。本研究旨在优化和验证简单序列重复（SSR）标记的聚合酶链反应条件，以评估该属的遗传多样性。从31份该属材料两周大的叶片中，使用改良的CTAB方案提取基因组DNA，对于难处理的样品则使用快速DNA植物/种子试剂盒（Zymo Research公司）。然后在优化条件下使用29个SSR标记对样品进行扩增。计算多态性标记的多态性信息含量（PIC）以确定其有效性。本研究确定，在25μL反应混合物中，dNTPs、MgCl和引物的最佳浓度分别为2.5、2和5 mM，DNA模板量为1μL，Taq酶量为0.125μL。平均PIC值为0.233，表明这些标记的信息含量较低。标记PC004是信息含量最高的标记，PIC值最高（0.605），尽管它是一个六核苷酸基序重复，但检测到的等位基因数量最多。其独特性增加了其在遗传多样性评估中的潜在用途。本研究表明，为该研究设计和优化的SSR标记对于该属物种的遗传多样性和群体结构分析、该属基因型的分子验证以及其他相关属具有重要意义。此外，研究结果为该属的遗传改良奠定了基础
