State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China; Research & Service Center for Environmental Industry, Yancheng 224051, Jiangsu Province, China.
Water Res. 2015 Apr 15;73:332-41. doi: 10.1016/j.watres.2015.01.041. Epub 2015 Feb 7.
Adding alkyl polyglucose (APG) into an anaerobic treatment system of waste activated sludge (WAS) was reported to remarkably improve the production of short-chain fatty acids (SCFAs), especially propionic acid via simultaneously accelerating solubilization and hydrolysis, enhancing acidification, inhibiting methanogenesis and balancing carbon to nitrogen (C/N) ratio of substrate. Not only the production of SCFAs, especially propionic acid, was significantly improved by APG, but also the feasible operation time was shortened. The SCFAs yield at 0.3 g APG per gram of total suspended solids (TSS) within 4 d was 2988 ± 60 mg chemical oxygen demand (COD) per liter, much higher than that those from sole WAS or sole WAS plus sole APG. The corresponding yield of propionic acid was 1312 ± 25 mg COD/L, 7.9-fold of sole WAS. Mechanism investigation showed that during anaerobic treatment of WAS in the presence of APG both the solubilization and hydrolysis were accelerated and the acidification was enhanced, while the methanogenesis was inhibited. Moreover, the activities of key enzymes involved in WAS hydrolysis and acidification were improved through the adjustment of C/N ratio of substrates with APG. The abundance of microorganisms responsible for organic compounds hydrolysis and SCFAs production was also observed to be greatly enhanced with APG via 454 high-throughput pyrosequencing analysis.
向厌氧处理废活性污泥（WAS）的系统中添加烷基多糖苷（APG），据报道可显著提高短链脂肪酸（SCFAs）的产量，特别是丙酸，这是通过同时加速溶解和水解、增强酸化、抑制产甲烷作用以及平衡底物的碳氮（C/N）比来实现的。APG 不仅显著提高了 SCFAs 的产量，特别是丙酸的产量，而且还缩短了可行的运行时间。在 4 天内，每克总悬浮固体（TSS）添加 0.3 克 APG 时，SCFAs 的产量为 2988 ± 60mg 化学需氧量（COD）/升，远高于单独的 WAS 或单独的 WAS 加单独的 APG。相应的丙酸产量为 1312 ± 25mg COD/L，是单独 WAS 的 7.9 倍。机理研究表明，在 APG 存在的情况下，WAS 的厌氧处理过程中，溶解和水解均得到加速，酸化得到增强，而产甲烷作用受到抑制。此外，通过 APG 调整底物的 C/N 比，还提高了参与 WAS 水解和酸化的关键酶的活性。通过 454 高通量焦磷酸测序分析还观察到，APG 大大增加了负责有机化合物水解和 SCFAs 产生的微生物的丰度。
