Zorumski C F, Todd R D, Clifford D B
Department of Psychiatry, Washington University School of Medicine, St. Louis 63110.
Brain Res. 1989 Aug 7;494(1):193-7. doi: 10.1016/0006-8993(89)90163-7.
The actions of the excitatory amino acid ibotenate were investigated in postnatal rat hippocampal neurons. In neurons voltage clamped at negative membrane potentials using low-chloride internal solutions, ibotenate responses consist of an inward cationic current and two outward currents. The inward current is inhibited by 2-amino-5-phosphonovalerate (APV), a selective N-methyl-D-aspartate (NMDA) antagonist. The two outward currents consist of a picrotoxin and bicuculline-sensitive chloride current and a slowly developing calcium activated potassium current. The bicuculline sensitive current appears to be the product of contamination of ibotenate samples with the gamma-aminobutyric acid (GABA) agonist muscimol and not the result of a direct action of ibotenate.
