Duarte-Barbosa Marcia, Bajay Miklos M, Zucchi Maria I, Pivello Vânia R
Department of Ecology, Universidade de São Paulo (USP), Rua do Matão 321, Travessa 14, Cidade Universitária, 05508-090 São Paulo, São Paulo, Brazil.
Department of Genetics, Universidade de São Paulo (USP), CP 83, Av. Pádua Dias 11, Vila Independência, 13400-970 Piracicaba, São Paulo, Brazil.
Appl Plant Sci. 2015 Feb 9;3(2). doi: 10.3732/apps.1400087. eCollection 2015 Feb.
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We developed and validated microsatellite primers for Vellozia squamata (Velloziaceae), an endemic species of the cerrado (Brazilian savannas), to investigate the influence of different fire regimes on its genetic diversity and population structure. •
Using a selective hybridization method, we tested 51 SSR loci using a natural population of V. squamata and obtained 47 amplifiable loci. Among these, 26 loci were polymorphic and the average values of genetic diversity were: average number of alleles per locus ([Formula: see text]) = 6.54, average number of alleles per polymorphic locus ([Formula: see text]) = 7.13, average observed heterozygosity [Formula: see text] = 0.22, average expected heterozygosity [Formula: see text] = 0.49, and average fixation index [Formula: see text] = 0.55. •
These 26 loci allowed us to assess the effects of distinct fire regimes on the genetic structure of V. squamata populations with the aim of establishing strategies for the conservation of this endemic species. The markers can also be useful for future pharmaceutical studies, as the species has great potential for medicinal and cosmetic applications.
研究前提：我们开发并验证了用于鳞叶木（Velloziaceae）的微卫星引物，该物种是塞拉多（巴西热带稀树草原）的特有物种，旨在研究不同火灾模式对其遗传多样性和种群结构的影响。
方法与结果：使用选择性杂交方法，我们对鳞叶木的一个自然种群测试了51个SSR位点，获得了47个可扩增位点。其中，26个位点具有多态性，遗传多样性的平均值为：每个位点的平均等位基因数（[公式：见正文]）= 6.54，每个多态性位点的平均等位基因数（[公式：见正文]）= 7.13，平均观察杂合度[公式：见正文]= 0.22，平均期望杂合度[公式：见正文]= 0.49，平均固定指数[公式：见正文]= 0.55。
结论：这26个位点使我们能够评估不同火灾模式对鳞叶木种群遗传结构的影响，目的是制定保护这种特有物种的策略。这些标记对于未来的药物研究也可能有用，因为该物种在药用和化妆品应用方面具有巨大潜力。
